Alrouji Mohammed, Kuriri Fahd A, Alqasmi Mohammed Hussein, AlSudais Hamood, Alissa Mohammed, Alsuwat Meshari A, Asad Mohammed, Joseph Babu, Almuhanna Yasir
Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, Shaqra University, Shaqra 11961, Saudi Arabia.
Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Saud University, Riyadh 12372, Saudi Arabia.
Microorganisms. 2023 Mar 8;11(3):692. doi: 10.3390/microorganisms11030692.
The study developed a simple and inexpensive method to induce biofilm formation in-vivo for the evaluation of the antibiofilm activity of pharmacological agents using Swiss albino mice. Animals were made diabetic using streptozocin and nicotinamide. A cover slip containing preformed biofilm along with MRSA culture was introduced into the excision wound in these animals. The method was effective in developing biofilm on the coverslip after 24 h incubation in MRSA broth which was confirmed by microscopic examination and a crystal violet assay. Application of preformed biofilm along with microbial culture induced a profound infection with biofilm formation on excision wounds in 72 h. This was confirmed by macroscopic, histological, and bacterial load determination. Mupirocin, a known antibacterial agent effective against MRSA was used to demonstrate antibiofilm activity. Mupirocin was able to completely heal the excised wounds in 19 to 21 days while in the base-treated group, healing took place between 30 and 35 days. The method described is robust and can be reproduced easily without the use of transgenic animals and sophisticated methods such as confocal microscopy.
该研究开发了一种简单且低成本的方法，用于在瑞士白化小鼠体内诱导生物膜形成，以评估药物制剂的抗生物膜活性。使用链脲佐菌素和烟酰胺使动物患糖尿病。将含有预先形成的生物膜以及耐甲氧西林金黄色葡萄球菌培养物的盖玻片引入这些动物的切除伤口中。在耐甲氧西林金黄色葡萄球菌肉汤中孵育24小时后，该方法可有效地在盖玻片上形成生物膜，这通过显微镜检查和结晶紫测定得到证实。将预先形成的生物膜与微生物培养物一起应用，在72小时内可在切除伤口上引发伴有生物膜形成的严重感染。这通过宏观、组织学和细菌载量测定得到证实。莫匹罗星是一种已知的对耐甲氧西林金黄色葡萄球菌有效的抗菌剂，用于证明其抗生物膜活性。莫匹罗星能够在19至21天内完全治愈切除的伤口，而在基础治疗组中，愈合发生在30至35天之间。所描述的方法可靠，无需使用转基因动物和共聚焦显微镜等复杂方法即可轻松重现。
