Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, Shaqra University, Shaqra 11961, Saudi Arabia.
Chair of Medical and Molecular Genetics Research, Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Saud University, Riyadh 12372, Saudi Arabia.
Int J Mol Sci. 2023 Jun 5;24(11):9774. doi: 10.3390/ijms24119774.
, a desert plant commonly known as yarrow, is traditionally used as an antimicrobial agent in folklore medicine in Saudi Arabia. The current study was undertaken to determine its antibiofilm activity against methicillin-resistant (MRSA) and multi-drug-resistant (MDR-) using in vitro and in vivo studies. A biofilm model induced through an excision wound in diabetic mice was used to evaluate its effect in vivo. The skin irritation and cytotoxic effects of the extract were determined using mice and HaCaT cell lines, respectively. The methanolic extract was analyzed with LC-MS to detect different phytoconstituents, which revealed the presence of 47 different phytoconstituents. The extract inhibited the growth of both tested pathogens in vitro. It also increased the healing of biofilm-formed excision wounds, demonstrating its antibiofilm, antimicrobial, and wound-healing action in vivo. The effect of the extract was concentration-dependent, and its activity was stronger against MRSA than MDR-. The extract formulation was devoid of a skin irritation effect in vivo and cytotoxic effect on HaCaT cell lines in vitro.
, 一种常见的沙漠植物，在沙特阿拉伯的传统民间医学中被用作一种抗菌剂。本研究旨在通过体外和体内研究来确定其对耐甲氧西林金黄色葡萄球菌（MRSA）和多药耐药菌（MDR-）的抗生物膜活性。通过糖尿病小鼠的切除伤口诱导生物膜模型来评估其体内效果。分别使用小鼠和 HaCaT 细胞系来确定提取物的皮肤刺激性和细胞毒性作用。使用 LC-MS 分析甲醇提取物以检测不同的植物成分，结果表明存在 47 种不同的植物成分。该提取物在体外抑制了两种受试病原体的生长。它还增加了生物膜形成的切除伤口的愈合，证明了其在体内的抗生物膜、抗菌和伤口愈合作用。提取物的作用具有浓度依赖性，其对 MRSA 的活性强于 MDR-。提取物配方在体内无皮肤刺激性，在体外对 HaCaT 细胞系也无细胞毒性。
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