Department of Botany & Microbiology, Faculty of Science, Alexandria University, Alexandria 21568, Egypt.
Department of Medical Laboratory Technology, Faculty of Applied Health Sciences Technology, Pharos University in Alexandria, Alexandria 21526, Egypt.
Molecules. 2023 Mar 7;28(6):2434. doi: 10.3390/molecules28062434.
The spread of antibiotic-resistant opportunistic microbes is a huge socioeconomic burden and a growing concern for global public health. In the current study, two endophytic fungal strains were isolated from roots and identified as MT597434.1 and KU324798.1. Secondary metabolites produced by and were extracted and tested for their antimicrobial activity. The highest activity was noticed against and treated with and secondary metabolites, respectively. crude extract was mainly composed of Pentadecanoic acid, 14-methyl-, methyl ester and 9-Octadecenoic acid ()-, methyl ester (26.66 and 18.01%, respectively), while crude extract's major components were 2,4-Decadienal, (,) and 9-Octadecenoic acid ()-, and methyl ester (10.69 and 10.32%, respectively). Moreover, a comparative study between the fungal extracts and dicationic pyridinium iodide showed that the combination of and secondary metabolites with dicationic pyridinium iodide compound showed a synergistic effect against . The combined formulae inhibited the bacterial growth after 4 to 6 h through cell wall breakage and cells deformation, with intracellular components leakage and increased ROS production.
抗生素耐药性机会性病原体的传播给社会经济带来了巨大负担，也成为了全球公共卫生日益关注的问题。在本研究中，从植物根部分离出两种内生真菌菌株，并将其鉴定为 MT597434.1 和 KU324798.1。从 和 中提取的次生代谢产物，并对其抗菌活性进行了测试。结果表明， 和 次生代谢产物对 和 表现出最高的活性。 粗提物主要由十五烷酸，14-甲基-，甲酯和 9-十八烯酸（），甲酯（分别为 26.66%和 18.01%）组成，而 粗提物的主要成分是 2,4-癸二烯醛（，）和 9-十八烯酸（），甲酯（分别为 10.69%和 10.32%）。此外，真菌提取物与二价吡啶碘化物的比较研究表明， 和 次生代谢产物与二价吡啶碘化物化合物的组合对 表现出协同作用。复合配方通过细胞壁破裂和细胞变形，导致细胞内成分泄漏和 ROS 产生增加，在 4 至 6 小时后抑制了细菌的生长。
