Agronomy of Food Science and Technology, Yanbian University, Yanji 133002, China.
Molecules. 2023 Mar 13;28(6):2591. doi: 10.3390/molecules28062591.
Silymarin and milk thistle oil have unique biological benefits; however, applying silymarin to milk thistle oil remains a challenge. In this research, the content of silymarin in milk thistle oil conditions using enzyme-mediated solvent extraction was investigated and optimized by response surface methodology. The optimal extraction conditions using enzyme-mediated solvent extraction were as follows: the enzyme-added content was 3.06 mg/mL, the enzymatic hydrolysis temperature was 55.09 °C, and the enzymatic hydrolysis time was 66.28 min. Oil extracted by the enzyme-mediated assisted solvent was further compared with those extracted with n-hexane and cold pressing. Results indicated that the oil extraction using the enzyme-mediated assisted solvent had a lower acid value (2.20 ± 0.01 mg/g) and the highest α-tocopherol content (0.62 ± 0.00 mg/g), total phenols (7.67 ± 0.01 mg/g), and flavonoids (1.06 ± 0.13 mg/g). Furthermore, the antioxidant capacity of milk thistle oils was further investigated. The results showed that the enzyme-mediated assisted solvent-extracted oil had the strongest antioxidant capacity with lower lipid oxide content. Therefore, enzyme-mediated solvent extraction is an excellent method for extracting milk thistle oil.
水飞蓟素和奶蓟油具有独特的生物学益处；然而，将水飞蓟素应用于奶蓟油仍然是一个挑战。在这项研究中，采用响应面法研究并优化了酶法辅助溶剂萃取对奶蓟油中水飞蓟素含量的影响。酶法辅助溶剂萃取的最佳提取条件为：加酶量 3.06mg/mL、酶解温度 55.09°C、酶解时间 66.28min。进一步将酶法辅助溶剂提取的油与正己烷提取的油和冷榨油进行比较。结果表明，酶法辅助溶剂提取的油具有较低的酸值（2.20±0.01mg/g）和较高的α-生育酚含量（0.62±0.00mg/g）、总酚（7.67±0.01mg/g）和类黄酮（1.06±0.13mg/g）。此外，还进一步研究了水飞蓟籽油的抗氧化能力。结果表明，酶法辅助溶剂提取的油具有最强的抗氧化能力，脂质氧化物含量较低。因此，酶法辅助溶剂萃取是一种提取奶蓟油的优秀方法。
