Laboratory of Materials, Molecules and Applications (LMMA), Preparatory Institute of Scientific and Technical Studies (IPEST), University of Carthage, La Marsa, 2070 Tunis, Tunisia.
Faculty of Science of Bizerte, Zarzouna, 7021 Bizerte, Tunisia.
Int J Mol Sci. 2017 Dec 2;18(12):2582. doi: 10.3390/ijms18122582.
In this study, milk thistle seeds growing in different areas in Tunisia were cold pressed and the extracted oils were examined for their chemical and antioxidant properties. The major fatty acids were linoleic acid (C18:2) (57.0%, 60.0%, and 60.3% for the milk thistle seed oils native to Bizerte, Zaghouan and Sousse, respectively) and oleic acid (C18:1) (15.5%, 21.5%, and 22.4% for the milk thistle seed oils originating from Bizerte, Zaghouan and Sousse, respectively). High performance liquid chromatography (HPLC) analysis showed the richness of the milk thistle seed oils (MTSO) in α-tocopherol. The highest content was recorded for that of the region of Zaghouan (286.22 mg/kg). The total phenolic contents (TPC) of Zaghouan, Bizerte, and Sousse were 1.59, 8.12, and 4.73 Gallic Acid Equivalent (GAE) mg/g, respectively. Three phenolic acids were also identified (vanillic, -coumaric, and silybine), with a predominance of the vanillic acid. The highest value was recorded for the Zaghouan milk thistle seed oil (83 mg/100 g). Differences in outcomes between regions may be due to climatic differences in areas. Zaghouan's cold-pressed milk thistle seed oil had a better quality than those of Bizerte and Sousse, and can be considered as a valuable source for new multi-purpose products or by-products for industrial, cosmetic, and pharmaceutical utilization.
在这项研究中，对突尼斯不同地区生长的乳蓟种子进行冷榨，研究了提取油的化学性质和抗氧化特性。主要脂肪酸为亚油酸（C18:2）（乳蓟种子油的含量分别为原产于比塞大、扎格万和苏塞的 57.0%、60.0%和 60.3%）和油酸（C18:1）（分别为原产于比塞大、扎格万和苏塞的 15.5%、21.5%和 22.4%）。高效液相色谱（HPLC）分析表明，乳蓟籽油富含α-生育酚。扎格万地区的含量最高（286.22mg/kg）。扎格万、比塞大和苏塞的总酚含量（TPC）分别为 1.59、8.12 和 4.73 没食子酸当量（GAE）mg/g。还鉴定了三种酚酸（香草酸、-香豆酸和水飞蓟宾），其中香草酸占主导地位。扎格万乳蓟籽油的含量最高（83mg/100g）。不同地区的结果差异可能是由于地区气候差异造成的。扎格万冷榨乳蓟籽油的品质优于比塞大和苏塞，可以考虑作为工业、化妆品和制药用途的新产品或副产品的有价值来源。
