Wang Linlin, Yang Feng, Rong Yali, Yuan Yuan, Ding Yatao, Shi Wenzheng, Wang Zhihe
College of Food Science and Technology Shanghai Ocean University Shanghai China.
National R&D Branch Center for Freshwater Aquatic Products Processing Technology (Shanghai) Shanghai China.
Food Sci Nutr. 2019 Jun 25;7(7):2224-2230. doi: 10.1002/fsn3.1017. eCollection 2019 Jul.
This study was investigated the effects of six proteases (papain, compound proteinase, acidic protease, neutrase, pancreatin, and alcalase) on the lipid yield and quality of krill oil. The result shown that the krill oil extracted by alcalase and compound proteinase led to comparatively higher lipid yields (5.29% and 4.90%, respectively), Content of tocopherols and vitamin A, the content of omega-3 polyunsaturated fatty acids (PUFAs) and phospholipids extracted by alcalase was relatively higher. Control and alcalase had comparatively higher concentration of astaxanthin. On the whole, compared with the extraction of solvent, enzymatic hydrolysis could improve the quality and the lipid yield of krill oil. Therefore, enzymatic hydrolysis could be used as a better method to extract krill oil.
本研究考察了六种蛋白酶（木瓜蛋白酶、复合蛋白酶、酸性蛋白酶、中性蛋白酶、胰酶和碱性蛋白酶）对磷虾油脂质产量和品质的影响。结果表明，用碱性蛋白酶和复合蛋白酶提取的磷虾油脂质产量相对较高（分别为5.29%和4.90%），碱性蛋白酶提取的生育酚和维生素A含量、ω-3多不饱和脂肪酸（PUFAs）和磷脂含量相对较高。对照组和碱性蛋白酶组的虾青素浓度相对较高。总体而言，与溶剂萃取相比，酶解可以提高磷虾油的品质和脂质产量。因此，酶解可作为一种更好的磷虾油提取方法。
