芦丁和槲皮素在暴露于辐射后是否保留其结构和自由基清除活性？

Do Rutin and Quercetin Retain Their Structure and Radical Scavenging Activity after Exposure to Radiation?

机构信息

Department of Pharmacognosy, Poznan University of Medical Sciences, Rokietnicka 3, 60-806 Poznan, Poland.

Department of Medicinal Chemistry, Medical University of Lublin, Jaczewskiego 4, 20-090 Lublin, Poland.

出版信息

Molecules. 2023 Mar 17;28(6):2713. doi: 10.3390/molecules28062713.

DOI:10.3390/molecules28062713

PMID:36985686

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10053567/

Abstract

The influence of ionizing radiation on the physicochemical properties of quercetin and rutin in the solid state was studied. Quercetin and rutin were irradiated with the standard recommended radiation dose (25 kGy) according to EN 522 standard. The samples were irradiated by electron beam radiation. EPR studies indicate the formation of a small number of free radicals due to irradiation. Moreover, some radicals recombined with the mean lifetime of 1200 and 93 h, and a stable radical concentration reached only 0.29 and 0.90 ppm for quercetin and rutin, respectively. The performed spectroscopic study (FT-IR) confirmed the radiostability of the flavonoids tested. Chromatographic tests (HPLC, HPLC-MS) showed that irradiation of quercetin and rutin with a 25 kGy dose did not change the physicochemical properties of the tested compounds. Degradation products were not observed. The antioxidant activities were determined by the 2,2-diphenyl-1-pycrylhydrazyl (DPPH) free radical scavenging activity assay, ABTS Radical Scavenging Assay (ABTS), Ferric Reducing Antioxidant Power Assay (FRAP), Cupric Ion Reducing Antioxidant Capacity Assay (CUPRAC). The conducted research confirmed that exposure to ionizing radiation does not change the chemical structure of tested flavonoids and their antioxidant properties.

摘要

研究了电离辐射对固态槲皮素和芦丁的物理化学性质的影响。根据 EN 522 标准，用标准推荐的辐射剂量（25 kGy）对槲皮素和芦丁进行辐照。样品用电子束辐射进行辐照。EPR 研究表明，由于辐照形成了少量自由基。此外，一些自由基在平均寿命为 1200 和 93 h 时重新组合，并且对于槲皮素和芦丁，稳定自由基浓度仅分别达到 0.29 和 0.90 ppm。进行的光谱研究（FT-IR）证实了所测试的类黄酮的辐射稳定性。色谱测试（HPLC、HPLC-MS）表明，用 25 kGy 剂量辐照槲皮素和芦丁不会改变测试化合物的物理化学性质。未观察到降解产物。抗氧化活性通过 2,2-二苯基-1-吡咯基肼（DPPH）自由基清除活性测定法、ABTS 自由基清除测定法（ABTS）、铁还原抗氧化能力测定法（FRAP）、铜离子还原抗氧化能力测定法（CUPRAC）来确定。进行的研究证实，暴露于电离辐射不会改变所测试类黄酮的化学结构及其抗氧化特性。