Liu Yifu, Xiao Wenfa, Wang Fude, Wang Ya, Dong Yao, Nie Wen, Tan Cancan, An Sanping, Chang Ermei, Jiang Zeping, Wang Junhui, Jia Zirui
Key Laboratory of Forest Ecology and Environment of National Forestry and Grassland Administration, Ecology and Nature Conservation Institute Chinese Academy of Forestry Beijing China.
State Key Laboratory of Tree Genetics and Breeding Chinese Academy of Forestry Beijing China.
Ecol Evol. 2024 Aug 6;14(8):e70126. doi: 10.1002/ece3.70126. eCollection 2024 Aug.
To study the interspecific differentiation characteristics of species originating from recent radiation, the genotyping-by-sequencing (GBS) technique was used to explore the kinship, population structure, gene flow, genetic variability, genotype-environment association and selective sweeps of complex with similar phenotypes from a genome-wide perspective. The following results were obtained: 14 populations of complex could be divided into 5 clades; and diverged earlier and were more distantly related to the remaining 6 spruce species. Various geological events have promoted the species differentiation of complex. There were four instances of gene flow among , , , and . The population of had the highest level of nucleotide diversity, and may have experienced a bottleneck recently. Genotype-environment association found that a total of 20,808 genes were related to the environmental variables, which enhanced the adaptability of spruce in different environments. Genes that were selectively swept in the complex were primarily associated with plant stress resistance. Among them were some genes involved in plant growth and development, heat stress, circadian rhythms and flowering. In addition to the commonly selected genes, different spruce species also displayed unique genes subjected to selective sweeps that improved their adaptability to different habitats. Understanding the interspecific gene flow and adaptive evolution of species is beneficial to further understanding the species relationships of spruce and can provide a basis for studying spruce introgression and functional genomics.
为研究近期辐射起源物种的种间分化特征，采用简化基因组测序（GBS）技术，从全基因组角度探讨了具有相似表型的云杉复合体的亲缘关系、群体结构、基因流、遗传变异性、基因型与环境关联以及选择性清除。得到以下结果：云杉复合体的14个群体可分为5个分支；[具体物种1]和[具体物种2]分化较早，与其余6种云杉的亲缘关系较远。各种地质事件促进了云杉复合体的物种分化。在[具体物种1]、[具体物种2]、[具体物种3]、[具体物种4]和[具体物种5]之间存在4次基因流事件。[具体物种6]群体的核苷酸多样性水平最高，[具体物种7]可能最近经历了瓶颈效应。基因型与环境关联分析发现，共有20808个基因与环境变量相关，这增强了云杉在不同环境中的适应性。云杉复合体中发生选择性清除的基因主要与植物抗逆性相关。其中包括一些参与植物生长发育、热应激、昼夜节律和开花的基因。除了常见的被选择基因外，不同云杉物种还显示出独特的经历选择性清除的基因，这些基因提高了它们对不同栖息地的适应性。了解云杉物种间的基因流和适应性进化，有助于进一步理解云杉的物种关系，并可为研究云杉渐渗和功能基因组学提供依据。
