Lee Jung-Woo, Kim Jang-Uk, Bang Kyong-Hwan, Kwon Nayeong, Kim Young-Chang, Jo Ick-Hyun, Park Young-Doo
Department of Herbal Crop Research, National Institution of Horticultural and Herbal Science, Rural Development Administration, Eumseong 27709, Republic of Korea.
Department of Horticultural Biotechnology, Kyung Hee University, Yongin 17104, Republic of Korea.
Plants (Basel). 2023 Mar 10;12(6):1270. doi: 10.3390/plants12061270.
Meyer grows in east Russia and Asia. There is a high demand for this crop due to its medicinal properties. However, its low reproductive efficiency has been a hindrance to the crop's widespread use. This study aims to establish an efficient regeneration and acclimatization system for the crop. The type of basal media and strength were evaluated for their effects on somatic embryogenesis, germination, and regeneration. The highest rate of somatic embryogenesis was achieved for the basal media MS, N6, and GD, with the optimal nitrogen content (≥35 mM) and NH/NO ratio (1:2 or 1:4). The full-strength MS medium was the best one for somatic embryo induction. However, the diluted MS medium had a more positive effect on embryo maturation. Additionally, the basal media affected shooting, rooting, and plantlet formation. The germination medium containing 1/2 MS facilitated good shoot development; however, the medium with 1/2 SH yielded outstanding root development. In vitro-grown roots were successfully transferred to soil, and they exhibited a high survival rate (86.3%). Finally, the ISSR marker analysis demonstrated that the regenerated plants were not different from the control. The obtained results provide valuable information for a more efficient micropropagation of various cultivars.
美叶小檗生长于俄罗斯东部和亚洲地区。由于其药用特性，这种作物的需求量很大。然而，其低繁殖效率一直是该作物广泛应用的障碍。本研究旨在建立一种高效的该作物再生与驯化体系。评估了基础培养基的类型和浓度对体细胞胚胎发生、萌发和再生的影响。基础培养基MS、N6和GD在最佳氮含量（≥35 mM）和铵态氮/硝态氮比例（1:2或1:4）时，体细胞胚胎发生率最高。全强度MS培养基是诱导体细胞胚胎的最佳培养基。然而，稀释的MS培养基对胚胎成熟有更积极的影响。此外，基础培养基对芽的生长、生根和植株形成有影响。含有1/2 MS的萌发培养基有利于芽的良好发育；然而，含有1/2 SH的培养基根系发育出色。在试管中生长的根成功移栽到土壤中，成活率很高（86.3%）。最后，ISSR标记分析表明，再生植株与对照无差异。所得结果为更高效地微繁殖各种品种提供了有价值的信息。
