Guan Yuan, Li Shui-Gen, Fan Xiao-Fen, Su Zhen-Hong
Forestry and Fruit Tree Research Institute, Shanghai Academy of Agricultural Sciences Shanghai, China.
Front Plant Sci. 2016 Jun 24;7:938. doi: 10.3389/fpls.2016.00938. eCollection 2016.
Somatic embryogenesis is a developmental process where a plant somatic cell can dedifferentiate to a totipotent embryonic stem cell that has the ability to give rise to an embryo under appropriate conditions. This new embryo can further develop into a whole plant. In woody plants, somatic embryogenesis plays a critical role in clonal propagation and is a powerful tool for synthetic seed production, germplasm conservation, and cryopreservation. A key step in somatic embryogenesis is the transition of cell fate from a somatic cell to embryo cell. Although somatic embryogenesis has already been widely used in a number of woody species, propagating adult woody plants remains difficult. In this review, we focus on molecular mechanisms of somatic embryogenesis and its practical applications in economic woody plants. Furthermore, we propose a strategy to improve the process of somatic embryogenesis using molecular means.
体细胞胚胎发生是一个发育过程,在此过程中植物体细胞可以去分化成为全能性胚胎干细胞,该细胞在适宜条件下能够发育成胚胎。这个新胚胎能够进一步发育成完整植株。在木本植物中,体细胞胚胎发生在克隆繁殖中起着关键作用,并且是用于合成种子生产、种质保存和超低温保存的有力工具。体细胞胚胎发生的一个关键步骤是细胞命运从体细胞向胚性细胞的转变。尽管体细胞胚胎发生已经在许多木本物种中得到广泛应用,但繁殖成年木本植物仍然困难。在本综述中,我们聚焦于体细胞胚胎发生的分子机制及其在经济木本植物中的实际应用。此外,我们提出了一种利用分子手段改进体细胞胚胎发生过程的策略。