Ozudogru Elif Aylin, Previati Alberto, Lambardi Maurizio
Department of Biology, Laboratory of Plant Tissue Culture, Gebze Institute of Technology, Kocaeli, Turkey.
Methods Mol Biol. 2010;589:303-24. doi: 10.1007/978-1-60327-114-1_28.
Today, the conservation of ornamental germplasm can take advantage of innovative techniques which allow preservation in vitro (slow growth storage) or in liquid nitrogen (cryopreservation) of plant material. Slow growth storage refers to the techniques enabling the in vitro conservation of shoot cultures in aseptic conditions by reducing markedly the frequency of periodic subculturing, without affecting the viability and regrowth of shoot cultures. Cryopreservation refers to the storage of explants from tissue culture at ultra-low temperature (-196 degrees C). At such temperature, all the biological reactions within the cells are hampered, hence the technique makes available the storage of plant material for theoretically unlimited periods of time. An exhaustive review of papers dealing with the slow growth storage and the cryopreservation of ornamental species is reported here. Step-by-step protocols for the slow growth storage of rose germplasm, the production of synthetic seeds for the in vitro conservation of ornamentals, and the cryopreservation of Chrysanthemum morifolium are included.
如今，观赏植物种质资源的保存可借助创新技术，这些技术能实现植物材料的离体保存（缓慢生长保存）或液氮保存（超低温保存）。缓慢生长保存是指通过显著降低定期继代培养的频率，在无菌条件下对茎尖培养物进行离体保存的技术，且不影响茎尖培养物的活力和再生能力。超低温保存是指将组织培养的外植体储存在超低温（-196℃）下。在这样的温度下，细胞内所有的生物反应都会受到抑制，因此该技术可使植物材料理论上能无限期保存。本文全面综述了有关观赏植物物种缓慢生长保存和超低温保存的论文。其中还包括玫瑰种质资源缓慢生长保存的分步方案、用于观赏植物离体保存的人工种子生产以及菊花的超低温保存。
