Clinical Medical College of Chengdu Medical College, Chengdu, Sichuan, China.
Department of Geriatric Medicine, The First Affiliated Hospital of Chengdu Medical College, Chengdu, Sichuan, China.
Front Endocrinol (Lausanne). 2023 Mar 13;14:1126129. doi: 10.3389/fendo.2023.1126129. eCollection 2023.
Leptin (LEP) acts as a proinflammatory cytokine and may play an important role in the pathophysiology of cystic fibrosis (CF). This review aimed to assess the quantitative difference in leptin status between CF patients and non-CF controls.
In this study, the researchers conducted systematic searches of various databases, such as PubMed, Excerpta Medica Database, Google Scholar, Web of Science, and the China National Knowledge Infrastructure. The data collected from the above databases were assessed using the Stata 11.0 and R 4.1.3 software. The correlation coefficients and the Standardized Mean Differences (SMD) were employed to assess the effect size. A combination analysis was also carried out with the help of either a fixed-effects or random-effects model. In addition, the single-cell sequencing GSE193782 dataset was obtained to determine the mRNA expression levels of LEP and leptin receptor (LEPR) in the bronchoalveolar lavage fluid, to verify the different leptin expression between the CF patients and healthy controls.
A total of 919 CF patients and 397 controls from 14 articles were included in this study. CF patients and non-CF controls showed similar serum/plasma leptin levels. Gender, specimen testing, age, and study design were all taken into account for carrying out subgroup analyses. The results revealed no variations in serum/plasma leptin levels between the controls and CF patients in the various subgroups. Female CF patients exhibited higher leptin concentrations compared to male CF patients, and male healthy individuals showed lower leptin levels than female healthy participants. Aside from the fact that serum/plasma leptin appeared to be favorably linked to fat mass and BMI, the findings in this study also indicated that serum/plasma concentrations were not associated with Forced Expiratory Volume in the first second (FEV1). No statistically significant differences were observed in the leptin and leptin receptor mRNA expression levels between the healthy controls and CF patients. The leptin receptor and leptin expression levels in alveolar lavage fluid were low in various cells, without any distinctive distribution patterns.
The current meta-analysis indicated the absence of significant differences in leptin levels between CF patients and healthy individuals. Gender, fat mass, and BMI may all be correlated with leptin concentrations.
https://www.crd.york.ac.uk/prospero/, identifier CRD42022380118.
瘦素（LEP）作为一种促炎细胞因子，可能在囊性纤维化（CF）的病理生理学中发挥重要作用。本研究旨在评估 CF 患者与非 CF 对照者之间瘦素状态的定量差异。
本研究对PubMed、Excerpta Medica Database、Google Scholar、Web of Science 和中国国家知识基础设施等多个数据库进行了系统检索。使用 Stata 11.0 和 R 4.1.3 软件评估上述数据库中收集的数据。使用相关系数和标准化均数差（SMD）来评估效应大小。还借助固定效应或随机效应模型进行了组合分析。此外，还获得了单细胞测序 GSE193782 数据集，以确定支气管肺泡灌洗液中 LEP 和瘦素受体（LEPR）的 mRNA 表达水平，从而验证 CF 患者和健康对照者之间的不同瘦素表达。
本研究共纳入了来自 14 篇文章的 919 名 CF 患者和 397 名对照者。CF 患者和非 CF 对照者的血清/血浆瘦素水平相似。考虑到性别、标本检测、年龄和研究设计，进行了亚组分析。结果显示，在各个亚组中，CF 患者和非 CF 对照者的血清/血浆瘦素水平没有差异。女性 CF 患者的瘦素浓度高于男性 CF 患者，而男性健康个体的瘦素水平低于女性健康参与者。除了血清/血浆瘦素似乎与脂肪量和 BMI 呈正相关之外，本研究还表明，血清/血浆浓度与第一秒用力呼气量（FEV1）无关。在健康对照者和 CF 患者之间，瘦素和瘦素受体的 mRNA 表达水平没有统计学上的显著差异。肺泡灌洗液中的瘦素受体和瘦素表达水平在各种细胞中均较低，且没有明显的分布模式。
本次荟萃分析表明 CF 患者与健康个体之间的瘦素水平没有显著差异。性别、脂肪量和 BMI 可能都与瘦素浓度有关。
https://www.crd.york.ac.uk/prospero/，标识符 CRD42022380118。
