Department of Microbiology and Immunology, University of Otago, Dunedin, New Zealand.
Genetics Otago, University of Otago, Dunedin, New Zealand.
Microb Genom. 2023 Mar;9(3). doi: 10.1099/mgen.0.000968.
sp. ATCC 39006 is a Gram-negative bacterium that has been used to study the function of phage defences, such as CRISPR-Cas, and phage counter-defence mechanisms. To expand our phage collection to study the phage-host interaction with sp. ATCC 39006, we isolated the T4-like myovirus LC53 in Ōtepoti Dunedin, Aotearoa New Zealand. Morphological, phenotypic and genomic characterization revealed that LC53 is virulent and similar to other , and phages belonging to the genus . Using a transposon mutant library, we identified the host gene as essential for phage infection, suggesting that it encodes the phage receptor. The genome of LC53 encodes all the characteristic T4-like core proteins involved in phage DNA replication and generation of viral particles. Furthermore, our bioinformatic analysis suggests that the transcriptional organization of LC53 is similar to that of phage T4. Importantly, LC53 encodes 18 tRNAs, which likely compensate for differences in GC content between phage and host genomes. Overall, this study describes a newly isolated phage infecting sp. ATCC 39006 that expands the diversity of phages available to study phage-host interactions.
sp. ATCC 39006 是一种革兰氏阴性细菌，已被用于研究噬菌体防御机制，如 CRISPR-Cas 和噬菌体反防御机制的功能。为了扩大我们的噬菌体收集，以研究与 sp. ATCC 39006 的噬菌体-宿主相互作用，我们从新西兰奥特罗阿新普利茅斯的Ōtepoti Dunedin 分离出 T4 样肌病毒 LC53。形态学、表型和基因组特征表明，LC53 是毒力相似的，类似于其他属于 属的 和 噬菌体。使用转座子突变体文库，我们确定了宿主 基因对噬菌体感染是必需的，这表明它编码噬菌体受体。LC53 的基因组编码所有参与噬菌体 DNA 复制和病毒颗粒生成的典型 T4 样核心蛋白。此外，我们的生物信息学分析表明，LC53 的转录组织与噬菌体 T4 相似。重要的是，LC53 编码 18 个 tRNA，这可能补偿了噬菌体和宿主基因组之间 GC 含量的差异。总的来说，这项研究描述了一种新分离的噬菌体感染 sp. ATCC 39006，这扩展了可用于研究噬菌体-宿主相互作用的噬菌体多样性。
