Wake Forest Institute for Regenerative Medicine, Wake Forest School of Medicine, Winston-Salem, NC, USA.
Department of Urology, Xinqiao Hospital, Third Military Medical University, Chongqing, People's Republic of China.
J Tissue Eng Regen Med. 2017 Feb;11(2):334-341. doi: 10.1002/term.1914. Epub 2014 Jun 19.
Stem cells are regarded as possible cell therapy candidates for skeletal muscle regeneration. However, invasive harvesting of those cells can cause potential harvest-site morbidity. The goal of this study was to assess whether human urine-derived stem cells (USCs), obtained through non-invasive procedures, can differentiate into skeletal muscle linage cells (Sk-MCs) and potentially be used for skeletal muscle regeneration. In this study, USCs were harvested from six healthy individuals aged 25-55. Expression profiles of cell-surface markers were assessed by flow cytometry. To optimize the myogenic differentiation medium, we selected two from four different types of myogenic differentiation media to induce the USCs. Differentiated USCs were identified with myogenic markers by gene and protein expression. USCs were implanted into the tibialis anterior muscles of nude mice for 1 month. The results showed that USCs displayed surface markers with positive staining for CD24, CD29, CD44, CD73, CD90, CD105, CD117, CD133, CD146, SSEA-4 and STRO-1, and negative staining for CD14, CD31, CD34 and CD45. After myogenic differentiation, a change in morphology was observed from 'rice-grain'-like cells to spindle-shaped cells. The USCs expressed specific Sk-MC transcripts and protein markers (myf5, myoD, myosin, and desmin) after being induced with different myogenic culture media. Implanted cells expressed Sk-MC markers stably in vivo. Our findings suggest that USCs are able to differentiate into the Sk-MC lineage in vitro and after being implanted in vivo. Thus, they might be a potential source for cell injection therapy in the use of skeletal muscle regeneration. Copyright © 2014 John Wiley & Sons, Ltd.
干细胞被认为是骨骼肌再生的潜在细胞治疗候选物。然而，这些细胞的侵入性采集会导致潜在的采集部位发病率。本研究的目的是评估是否可以通过非侵入性程序从人尿中获得的干细胞（USC）分化为骨骼肌谱系细胞（Sk-MC），并可潜在用于骨骼肌再生。在这项研究中，从 6 名年龄在 25-55 岁的健康个体中采集 USC。通过流式细胞术评估细胞表面标志物的表达谱。为了优化成肌分化培养基，我们从 4 种不同类型的成肌分化培养基中选择了两种来诱导 USC。通过基因和蛋白表达鉴定分化的 USC 具有成肌标志物。将 USC 植入裸鼠的胫骨前肌中 1 个月。结果表明，USC 表面标志物呈阳性染色，表达 CD24、CD29、CD44、CD73、CD90、CD105、CD117、CD133、CD146、SSEA-4 和 STRO-1，阴性染色 CD14、CD31、CD34 和 CD45。在成肌分化后，观察到细胞形态从“米粒”样细胞变为梭形细胞。用不同的成肌培养基诱导后，USC 表达特定的 Sk-MC 转录本和蛋白标志物（myf5、myoD、肌球蛋白和结蛋白）。植入细胞在体内稳定表达 Sk-MC 标志物。我们的研究结果表明，USC 能够在体外分化为 Sk-MC 谱系，并在体内植入后分化。因此，它们可能是骨骼肌再生细胞注射治疗的潜在来源。版权所有©2014 约翰威立父子有限公司
