Węglewska Martyna, Barylski Jakub, Wojnarowski Filip, Nowicki Grzegorz, Łukaszewicz Marcin
Department of Molecular Virology, Faculty of Biology, Adam Mickiewicz University, Poznań, Poland.
GenXone INC, Poznań, Poland.
Front Microbiol. 2023 Mar 14;14:1120147. doi: 10.3389/fmicb.2023.1120147. eCollection 2023.
Bacteriophages from the subfamily ( family) have proven to be effective against bacteria from the genus including organisms from the group, which cause food poisoning and persistent contamination of industrial installations. However, successful application of these phages in biocontrol depends on understanding of their biology and stability in different environments. In this study, we isolated a novel virus from garden soil in Wrocław (Poland) and named it 'Thurquoise'. The genome of that phage was sequenced and assembled into a single continuous contig with 226 predicted protein-coding genes and 18 tRNAs. The cryo-electron microscopy revealed that Thurquoise has complex virion structure typical for the family. Confirmed hosts include selected bacteria from the group-specifically (isolation host) and , but susceptible strains display different efficiency of plating (EOP). The eclipse and latent periods of Thurquoise in the isolation host last ~ 50 min and ~ 70 min, respectively. The phage remains viable for more than 8 weeks in variants of the SM buffer with magnesium, calcium, caesium, manganese or potassium and can withstand numerous freeze-thaw cycles if protected by the addition of 15% glycerol or, to a lesser extent, 2% gelatine. Thus, with proper buffer formulation, this virus can be safely stored in common freezers and refrigerators for a considerable time. The Thurquoise phage is the exemplar of a new candidate species within the genus in the subfamily of the family with a genome, morphology and biology typical for these taxa.
来自该亚科（科）的噬菌体已被证明对来自该属的细菌有效，包括来自该组的生物体，这些生物体可导致食物中毒和工业设施的持续污染。然而，这些噬菌体在生物防治中的成功应用取决于对其生物学特性和在不同环境中的稳定性的了解。在本研究中，我们从弗罗茨瓦夫（波兰）的花园土壤中分离出一种新型病毒，并将其命名为“绿松石”。对该噬菌体的基因组进行了测序，并组装成一个单一的连续重叠群，其中有226个预测的蛋白质编码基因和18个tRNA。冷冻电子显微镜显示，绿松石具有该科典型的复杂病毒粒子结构。已确认的宿主包括来自该组的选定细菌——特别是（分离宿主）和，但敏感菌株表现出不同的平板接种效率（EOP）。绿松石在分离宿主中的隐蔽期和潜伏期分别持续约50分钟和约70分钟。该噬菌体在含有镁、钙、铯、锰或钾的SM缓冲液变体中可存活超过8周，如果添加15%甘油或在较小程度上添加2%明胶进行保护，它可以承受多次冻融循环。因此，通过适当的缓冲液配方，这种病毒可以在普通冰箱和冰柜中安全储存相当长的时间。绿松石噬菌体是该科亚科该属内一个新候选物种的典范，具有这些分类群典型的基因组、形态和生物学特性。
