Song Rongzhen, Li Wen, Deng Shanggui, Zhao Yueliang, Tao Ningping
College of Food Science and Technology, Shanghai Ocean University, Shanghai, China.
College of Food and Pharmacy, Zhejiang Ocean University, Zhoushan, Zhejiang, China.
Front Nutr. 2023 Mar 14;10:1136490. doi: 10.3389/fnut.2023.1136490. eCollection 2023.
In this study, we analyzed the eicosapentaenoic acid/docosahexaenoic acid (EPA/DHA) lipid composition of fish oil obtained through enzymatic treatment, fractional distillation and silica gel column purification, and further assessed EPA/DHA bioavailability. Lipid subclass composition information was obtained through ultra-performance liquid chromatography-electrospray ionization tandem mass spectrometry (UPLC-ESI-MS/MS), and bioavailability tests were performed using the Caco-2 cell monolayer model. Results showed that enzymatic treatment improved the incorporation of EPA/DHA as diacylglycerol (DG) while silica gel column chromatography enriched the content of EPA/DHA as phosphatidylglycerol (PG) (12.58%) and phosphatidylethanolamine (PE) (4.99%). Furthermore, increasing the purity of EPA/DHA could improve its bioavailability and after 24 incubation, binding forms of triglyceride (TG) was superior to ethyl ester (EE) ( < 0.05) at the same purity level. Those findings are helpful to provide research basis for exploring the bioactivity of fish oil.
在本研究中，我们分析了通过酶处理、分馏和硅胶柱纯化获得的鱼油中二十碳五烯酸/二十二碳六烯酸（EPA/DHA）的脂质组成，并进一步评估了EPA/DHA的生物利用度。通过超高效液相色谱-电喷雾电离串联质谱（UPLC-ESI-MS/MS）获得脂质亚类组成信息，并使用Caco-2细胞单层模型进行生物利用度测试。结果表明，酶处理提高了EPA/DHA作为二酰基甘油（DG）的掺入量，而硅胶柱色谱法富集了EPA/DHA作为磷脂酰甘油（PG）（12.58%）和磷脂酰乙醇胺（PE）（4.99%）的含量。此外，提高EPA/DHA的纯度可以提高其生物利用度，在相同纯度水平下孵育24小时后，甘油三酯（TG）的结合形式优于乙酯（EE）（<0.05）。这些发现有助于为探索鱼油的生物活性提供研究依据。
