Reuben C, Sundaram K, Phondke G P
Immunology. 1979 Jan;36(1):159-64.
The effect of antilymphocyte serum (ALS) on the secondary humoral immune response to sheep erythrocytes (SRBC) in rats was studied by the Jerne plaque assay technique. Its effect was also studied on the delayed hypersensitivity (DH) response to SRBC by the foot pad swelling test. ALS(N), which was prepared against lymphocytes from normal rats, had no effect on the secondary humoral and cellular response or on the primary cellular response, when administered postantigenically. ALS(I), which was raised against lymph node cells from SRBC immunized rats produced significant immunosuppression of the secondary response to SRBC when administered either before or after the antigenic injections. In the case of DH, ALS(I) behaved just like ALS(N) having no effect on the secondary response and suppressing the primary only when administered prior to the antigen.
采用耶尔恩氏空斑试验技术研究了抗淋巴细胞血清(ALS)对大鼠针对绵羊红细胞(SRBC)的二次体液免疫反应的影响。还通过足垫肿胀试验研究了其对针对SRBC的迟发型超敏反应(DH)的影响。针对正常大鼠淋巴细胞制备的ALS(N),在抗原注射后给予时,对二次体液和细胞反应或初次细胞反应均无影响。针对经SRBC免疫的大鼠淋巴结细胞制备的ALS(I),在抗原注射前或后给予时,均对针对SRBC的二次反应产生显著的免疫抑制作用。就DH而言,ALS(I)的表现与ALS(N)相似,对二次反应无影响,仅在抗原注射前给予时抑制初次反应。