Danley D L, Hilger A E, Boonlayangoor P, Millhouse E W
Infect Immun. 1979 Jan;23(1):115-27. doi: 10.1128/iai.23.1.115-127.1979.
Spleen cells from mice immunized with a variety of antigens and incubated in vitro with killed spherules of Coccidioides immitis lyse six to eight times more autologous murine erythrocytes than normal spleen cells and spherules. Cellular and biochemical events in this phenomenon were investigated to ascertain its significance. Kinetic studies suggested that hemolysis results from the activation of some immune cells by spherules. The capacity of spherules to activate these cells is rather unusual because, of the inert particles tested, only zymosan A and crude chitin demonstrated comparable activity. Furthermore, although the hemolytic phenomenon occurred in serum-free medium, more lysis was effected by immune cells and opsonized spherules or zymosan A than by immune cells and untreated fungal particles. Sheep, chicken, and human erythrocytes were not lysed in the hemolytic phenomenon; however, hemoglobin in chicken and sheep erythrocytes was oxidized. Both the murine erythrocyte lysis and oxidation of ovine hemoglobin correlated with the reduction of Nitro Blue Tetrazolium by immune cells adherent to spherules, and both phenomena appeared to be mediated by H2O2 released into the medium by activated cells. Spleen cells reactive with spherules could not be depleted by treatment with iron carbonyl, antiimmunoglobulin plus complement, or anti-brain-associated theta plus complement, but they were partially or completely depleted after rosette formation with erythrocytes coated with antibody or murine complement. Using light and electron microscopy, we noted that immune spleens contained more neutrophils than normal spleens, that these neutrophils reduced Nitro Blue Tetrazolium after stimulation with phorbol myristate acetate, and that they were the most prevalent cell type adherent to spherules after incubation in vitro.
用多种抗原免疫的小鼠的脾细胞，在体外与粗球孢子菌的灭活小球一起孵育，其溶解自体鼠红细胞的能力比正常脾细胞和小球高6至8倍。对这一现象中的细胞和生化事件进行了研究，以确定其意义。动力学研究表明，溶血是由小球激活某些免疫细胞所致。小球激活这些细胞的能力相当独特，因为在所测试的惰性颗粒中，只有酵母聚糖A和粗几丁质表现出类似的活性。此外，尽管溶血现象发生在无血清培养基中，但与免疫细胞和未处理的真菌颗粒相比，免疫细胞和调理过的小球或酵母聚糖A引起的细胞溶解更多。在溶血现象中，绵羊、鸡和人的红细胞未被溶解；然而，鸡和绵羊红细胞中的血红蛋白被氧化。鼠红细胞溶解和羊血红蛋白氧化均与附着在小球上的免疫细胞对硝基蓝四氮唑的还原有关，这两种现象似乎都是由活化细胞释放到培养基中的过氧化氢介导的。与小球反应的脾细胞不能通过用羰基铁、抗免疫球蛋白加补体或抗脑相关θ加补体处理而被耗尽，但在用抗体或鼠补体包被的红细胞形成花环后，它们会部分或完全被耗尽。通过光学和电子显微镜观察，我们发现免疫脾中的中性粒细胞比正常脾中的多，这些中性粒细胞在用佛波酯刺激后能还原硝基蓝四氮唑，并且在体外孵育后，它们是附着在小球上最普遍的细胞类型。
