解脲脲原体的连续培养
Cultivation of Ureaplasma urealyticum in continuous culture.
作者信息
Masover G K, Perez R, Matin A
出版信息
Infect Immun. 1979 Jan;23(1):175-7. doi: 10.1128/iai.23.1.175-177.1979.
Abstract
Continuous culture of Ureaplasma urealyticum is reported with a steady-state cell biomass of greater than 10(6) cells per ml. Thus, large cell numbers can be easily obtained; in addition, the system provides a powerful means for exploring what nutrients(s) limits the growth yield of this organism. Urea is shown not to be the growth-limiting nutrient in conventional media, although when provided in excess it appears to be completely hydrolyzed.
摘要
据报道，解脲脲原体的连续培养可获得每毫升大于10⁶个细胞的稳定细胞生物量。因此，可以轻松获得大量细胞；此外，该系统为探索何种营养物质限制该生物体的生长产量提供了有力手段。结果表明，在传统培养基中尿素并非生长限制营养物质，尽管过量提供时它似乎会被完全水解。
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本文引用的文献
1
Occurrence of urease in T strains of Mycoplasma.脲酶在支原体T株中的存在情况。
J Bacteriol. 1967 May;93(5):1513-20. doi: 10.1128/jb.93.5.1513-1520.1967.
2
Influence of urea on the growth of T-strain mycoplasmas.尿素对T株支原体生长的影响。
J Bacteriol. 1967 May;93(5):1509-12. doi: 10.1128/jb.93.5.1509-1512.1967.
3
Tissue cultures and mycoplasmas.组织培养与支原体。
Tex Rep Biol Med. 1965 Jun;23:Suppl 1:285+.
4
Growth of T-strain mycoplasmas in medium without added urea: effect of trace amounts of urea and of a urease inhibitor.T株支原体在未添加尿素的培养基中的生长：微量尿素和脲酶抑制剂的作用
J Bacteriol. 1974 Feb;117(2):765-74. doi: 10.1128/jb.117.2.765-774.1974.
5
"On being the right size".论恰到好处的尺寸
Annu Rev Microbiol. 1973;27:119-32. doi: 10.1146/annurev.mi.27.100173.001003.
6
Continuous culture used for media optimization.连续培养用于培养基优化。
Appl Microbiol. 1974 Dec;28(6):901-5. doi: 10.1128/am.28.6.901-905.1974.
7
Active transport of amino acids by membrane vesicles of Thiobacillus neapolitanus.那不勒斯硫杆菌膜囊泡对氨基酸的主动运输
J Gen Microbiol. 1974 Aug;83(2):311-8. doi: 10.1099/00221287-83-2-311.
8
Effect of urea concentration on growth of Ureaplasma urealyticum (T-strain mycoplasma).尿素浓度对解脲脲原体（T株支原体）生长的影响。
J Bacteriol. 1977 Oct;132(1):144-50. doi: 10.1128/jb.132.1.144-150.1977.
9
Some effects of growth medium composition on the antigenicity of a T-strain mycoplasma.生长培养基成分对T株支原体抗原性的一些影响。
Infect Immun. 1975 Mar;11(3):530-9. doi: 10.1128/iai.11.3.530-539.1975.
10
Physiological basis of the selective advantage of a Spirillum sp. in a carbon-limited environment.一种螺旋菌属细菌在碳限制环境中选择性优势的生理基础。
J Gen Microbiol. 1978 Apr;105(2):187-97. doi: 10.1099/00221287-105-2-187.
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