T株支原体在未添加尿素的培养基中的生长：微量尿素和脲酶抑制剂的作用
Growth of T-strain mycoplasmas in medium without added urea: effect of trace amounts of urea and of a urease inhibitor.
作者信息
Masover G K, Benson J R, Hayflick L
出版信息
J Bacteriol. 1974 Feb;117(2):765-74. doi: 10.1128/jb.117.2.765-774.1974.
Abstract
Urea is currently considered to be a requirement for the propagation of T-strain mycoplasmas. We report here the replication of T-strain 960 (ATCC 25023) in media prepared from dialyzed components with added putrescine and allantoin but without added urea, or in dialyzed medium containing small amounts of added urea. The least amount of urea which allowed growth in the medium without allantoin was above 10 mug/ml. The amount of urea estimated to contaminate the added allantoin or putrescine was 5 mug/ml or less, which is insufficient to support T-strain replication. T-strain 960 was grown in the presence of urea and the urease inhibitor acetohydroxamic acid AHA where the organisms multiplied at a slower rate in the presence of AHA than in its absence. Urea hydrolysis occurred with concomitant ammonia accumulation and pH increase in cultures with AHA added.
摘要
目前认为尿素是T株支原体繁殖所必需的。我们在此报告T株960（ATCC 25023）在由添加了腐胺和尿囊素但未添加尿素的透析成分制备的培养基中，或在含有少量添加尿素的透析培养基中的复制情况。在不含尿囊素的培养基中允许生长的最低尿素量高于10微克/毫升。估计污染添加的尿囊素或腐胺的尿素量为5微克/毫升或更少，不足以支持T株的复制。T株960在尿素和脲酶抑制剂乙酰氧肟酸（AHA）存在的情况下生长，在有AHA存在时，生物体的繁殖速度比没有AHA时慢。添加AHA的培养物中发生尿素水解，同时伴有氨积累和pH值升高。
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本文引用的文献
1
Sorbic Hydroxamic Acid, an Antifungal Agent Effective over a Wide pH Range.山梨基异羟肟酸，一种在广泛pH范围内有效的抗真菌剂。
Appl Microbiol. 1963 Jul;11(4):362-4. doi: 10.1128/am.11.4.362-364.1963.
2
A microdiffusion method for the determination of nitrogen liberated as ammonia.一种用于测定以氨形式释放的氮的微扩散法。
J Lab Clin Med. 1951 Aug;38(2):324-30.
3
UREASE CATALYSIS. II. INHIBITION OF THE ENZYME BY HYDROXYUREA, HYDROXYLAMINE, AND ACETOHYDROXAMIC ACID.脲酶催化作用。II. 羟基脲、羟胺和乙酰氧肟酸对该酶的抑制作用。
J Biol Chem. 1965 Jun;240:2407-14.
4
Specific inhibition of urease by hydroxamic acids.异羟肟酸对脲酶的特异性抑制作用。
Biochim Biophys Acta. 1962 Dec 4;65:380-3. doi: 10.1016/0006-3002(62)91067-3.
5
T-Form colonies of pleuropneumonialike organisms.胸膜肺炎样微生物的T形菌落。
J Bacteriol. 1956 Mar;71(3):362-9. doi: 10.1128/jb.71.3.362-369.1956.
6
The recovery of pleuropneumonia-like organisms from Negro men with and without nongonococcal urethritis.从患有和未患有非淋菌性尿道炎的黑人男性中分离出胸膜肺炎样微生物。
Am J Syph Gonorrhea Vener Dis. 1954 Mar;38(2):113-24.
7
Some properties and purifications of urease.
Biochim Biophys Acta. 1967 Sep 12;146(1):205-18. doi: 10.1016/0005-2744(67)90087-3.
8
Occurrence of urease in T strains of Mycoplasma.脲酶在支原体T株中的存在情况。
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9
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J Bacteriol. 1966 Jul;92(1):6-12. doi: 10.1128/jb.92.1.6-12.1966.
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