Karamon Jacek, Samorek-Pieróg Małgorzata, Bilska-Zając Ewa, Korpysa-Dzirba Weronika, Sroka Jacek, Zdybel Jolanta, Cencek Tomasz
Department of Parasitology and Invasive Diseases, National Veterinary Research Institute, 24-100 Puławy, Poland.
J Vet Res. 2024 Nov 6;68(4):539-549. doi: 10.2478/jvetres-2024-0060. eCollection 2024 Dec.
The aim of this study was to estimate the occurrence of spp. and other helminth infections in grey wolves in south-eastern Poland.
Overall, 74 samples of wolf faeces were examined with a multiplex PCR and a system of real-time quantitative PCR methods to detect and identify spp. The faeces were additionally examined microscopically. Also, 20 samples of wolf intestines were examined with a sedimentation and counting technique (SCT).
DNA was detected in 6.8% and . (identified as ) in 4.1% of faeces samples. spp. DNA was found in 43.2% and in 4.1%. Examination of the intestines by SCT showed worms in 10%, . () in10%, spp. in 100%, hookworms in 30%, in 20%, sp. in 10%, in 15%, sp. in 5% and sp. in 5%. By coproscopy, Capillariidae eggs were found in 59% of faeces samples. Genetic analysis of worms showed the presence of two European haplotypes previously described in Poland in red foxes and pigs. Sequences of obtained from worms shared full identity with a sequence from a human case in Poland.
The study showed the presence of in wolves for the first time in Poland and confirmed our earlier observations on . This double threat from in this wolf population should be taken into account when assessing the epidemiological risk. The study enriched the knowledge of other helminths found in wolves, also those () that were recorded for the first time in this species.
本研究的目的是估计波兰东南部灰狼体内 spp. 和其他蠕虫感染的发生率。
总共对74份狼粪便样本采用多重聚合酶链反应（PCR）和实时定量PCR方法系统进行检测和鉴定 spp.。此外，对粪便进行显微镜检查。同时，对20份狼肠道样本采用沉淀计数技术（SCT）进行检查。
在6.8%的粪便样本中检测到DNA，在4.1%的粪便样本中鉴定为 （具体物种未明确）。在43.2%的粪便样本中发现了 spp. DNA，在4.1%的样本中发现了 （具体物种未明确）。通过SCT对肠道进行检查发现，10%的样本中有 虫，10%的样本中有 （具体物种未明确），100%的样本中有 spp.，30%的样本中有钩虫，20%的样本中有 （具体物种未明确），10%的样本中有 sp.，15%的样本中有 （具体物种未明确），5%的样本中有 sp.，5%的样本中有 sp.。通过粪便检查，在59%的粪便样本中发现了毛细科虫卵。对 虫的基因分析显示存在两种先前在波兰红狐和猪中描述过的欧洲单倍型。从 虫获得的 序列与波兰一例人类病例的序列完全相同。
该研究首次在波兰的狼体内发现了 ，并证实了我们之前对 的观察结果。在评估该狼群的流行病学风险时，应考虑到 带来的双重威胁。该研究丰富了关于狼体内发现的其他蠕虫的知识，包括首次在该物种中记录到的那些（具体物种未明确）。
