Baty J D, Lindsay R M, Sharp S
J Chromatogr. 1986 Feb 26;353:329-37. doi: 10.1016/s0021-9673(01)87103-x.
Liquid chromatographic methods were developed for the study of the in vitro acetylation of the sulphonamide drug sulphamethazine and a series of aniline derivatives. The sensitivity of the methods have allowed data on the activity of the N-acetyltransferase enzyme(s) in man to be obtained. The use of bonded-phase columns with a series of organic mobile phases has been compared with reversed-phase separation of the acetanilide derivatives, produced in the acetylation reaction.
开发了液相色谱法用于研究磺胺类药物磺胺二甲嘧啶及一系列苯胺衍生物的体外乙酰化反应。这些方法的灵敏度使得能够获得关于人体中N - 乙酰转移酶活性的数据。已将使用带有一系列有机流动相的键合相柱与乙酰化反应中生成的乙酰苯胺衍生物的反相分离进行了比较。
