土曲霉喜树堿-海藻酸钠/二氧化钛纳米粒子作为一种新型纳米复合材料，具有增强的体内相容性和抗癌效率。

Aspergillus terreus camptothecin-sodium alginate/titanium dioxide nanoparticles as a novel nanocomposite with enhanced compatibility and anticancer efficiency in vivo.

机构信息

Department of Physics, Faculty of Science, Zagazig University, PO 44519, Zagazig, Egypt.

Enzymology and Fungal Biotechnology Lab, Botany and Microbiology Department, Faculty of Science, Zagazig University, Zagazig, 44519, Egypt.

出版信息

BMC Biotechnol. 2023 Apr 1;23(1):9. doi: 10.1186/s12896-023-00778-6.

DOI:10.1186/s12896-023-00778-6

PMID:37005635

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10067238/

Abstract

BACKGROUND

Camptothecin derivatives are one of the most prescribed anticancer drugs for cancer patients, however, the availability, efficiency, and water solubility are the major challenges that halt the applicability of this drug.

METHODS

Biosynthetic potency of camptothecin by Aspergillus terreus, open a new avenue for commercial camptothecin production, due to their short-life span, feasibility of controlled growth conditions, and affordability for higher growth, that fulfill the availability of the scaffold of this drug.

RESULTS

Camptothecin (CPT) was purified from the filtrates of A. terreus, and their purity was checked by HPLC, and its chemical structure was verified by LC/MS, regarding to the authentic one. To improve the anticancer efficiency of A. terreus CPT, the drug was conjugated with sodium alginate (SA)/Titanium dioxide nanoparticles (TiONPs) composites, and their physicochemical properties were assessed. From the FT-IR profile, a numerous hydrogen bond interactions between TiO and SA chains in the SA/TiO nanocomposites, in addition to the spectral changes in the characteristic bands of both SA/TiO and CPT that confirmed their interactions. Transmission electron microscopy analysis reveals the spherical morphology of the developed SA/TiONPs nanocomposite, with the average particle size ~ 13.3 ± 0.35 nm. From the results of zeta potential, successful loading and binding of CPT with SA/TiO nanocomposites were observed.

CONCLUSION

The in vivo study authenticates the significant improvement of the antitumor activity of CPT upon loading in SA/TiO nanocomposites, with affordable stability of the green synthesized TiONPs with Aloe vera leaves extract.

摘要

背景

喜树碱衍生物是癌症患者最常开的抗癌药物之一，但是其可用性、效率和水溶性是阻止该药物应用的主要挑战。

方法

土曲霉产生喜树碱的生物合成能力为商业喜树碱生产开辟了新途径，因为它们的寿命短、生长条件可控、生长成本高，满足了该药物支架的可用性。

结果

喜树碱（CPT）从土曲霉的滤液中纯化出来，并用 HPLC 检查其纯度，并通过 LC/MS 验证其化学结构与标准品一致。为了提高土曲霉 CPT 的抗癌效率，将该药物与海藻酸钠（SA）/二氧化钛纳米粒子（TiONPs）复合材料偶联，并评估其物理化学性质。从傅里叶变换红外（FT-IR）图谱可以看出，SA/TiO 纳米复合材料中 TiO 和 SA 链之间存在大量氢键相互作用，以及 SA/TiO 和 CPT 的特征带谱发生变化，证实了它们的相互作用。透射电子显微镜分析显示，所开发的 SA/TiONPs 纳米复合材料具有球形形态，平均粒径约为 13.3±0.35nm。从zeta 电位的结果可以看出，CPT 与 SA/TiO 纳米复合材料的成功负载和结合。