De Silva N S, Quinn P A
J Clin Microbiol. 1986 Feb;23(2):354-9. doi: 10.1128/jcm.23.2.354-359.1986.
The results of recent studies support the concept that Ureaplasma urealyticum may be a major cause of perinatal infection in both term and preterm infants. It has been postulated that phospholipase degradation of placental phospholipids by microorganisms triggers the onset of premature labor. Since the presence of ureaplasmas in placentas is associated with pregnancy loss, prematurity, and neonatal morbidity, we assayed U. urealyticum for the presence of phospholipase A1, A2, and C activities. Phospholipase A1 activity was low in lysates of exponential-phase cells of U. urealyticum. Phospholipase A2 activity was present and was 100-fold higher than the activity of phospholipase A1 in serotypes 3,4, and 8. The total activity and specific activity of phospholipase A2 in serotype 8 were nearly threefold higher than the activities in serotypes 3 and 4. Cell lysates of all three serotypes showed the presence of phospholipase C activity during the exponential phase of growth, and no significant difference in activity was observed among the three serotypes. In stationary-phase cells the phospholipase C activity was 10-fold lower than the activity in exponential-phase cells. Our results demonstrate that phospholipase activities are present in U. urealyticum cells and that the specific activities of phospholipase A2 differed among the three serotypes tested, while the activities of phospholipases A1 and C were similar.
解脲脲原体可能是足月和早产婴儿围产期感染的主要原因。据推测，微生物对胎盘磷脂的磷脂酶降解会引发早产。由于胎盘存在脲原体与妊娠丢失、早产和新生儿发病相关，我们检测了解脲脲原体中磷脂酶A1、A2和C的活性。解脲脲原体指数生长期细胞裂解物中的磷脂酶A1活性较低。磷脂酶A2活性存在，且在血清型3、4和8中比磷脂酶A1的活性高100倍。血清型8中磷脂酶A2的总活性和比活性比血清型3和4中的活性高近三倍。所有三种血清型的细胞裂解物在生长指数期均显示存在磷脂酶C活性，且三种血清型之间未观察到活性有显著差异。在稳定期细胞中，磷脂酶C活性比指数生长期细胞中的活性低10倍。我们的结果表明，解脲脲原体细胞中存在磷脂酶活性，所检测的三种血清型中磷脂酶A2的比活性不同，而磷脂酶A1和C的活性相似。
