用于检测解脲脲原体血清型特异性抗体的酶联免疫吸附测定
Enzyme-linked immunosorbent assay for detection of specific antibodies to Ureaplasma urealyticum serotypes.
作者信息
Wiley C A, Quinn P A
出版信息
J Clin Microbiol. 1984 Mar;19(3):421-6. doi: 10.1128/jcm.19.3.421-426.1984.
Abstract
Optimal conditions of a micro-enzyme-linked immunosorbent assay system for the detection of immunoglobulin G antibodies to Ureaplasma urealyticum were established with rabbit antisera. Initially, the antisera, raised against eight U. urealyticum serotypes grown on medium containing horse serum, displayed nonspecific reactions with our enzyme-linked immunosorbent assay antigens. Substitution of fetal bovine serum in the medium eliminated this nonspecificity. The assay was then serotype-specific for the original eight U. urealyticum serotypes. The prominent homologous reaction was easily differentiated from the heterologous reactions. A one-way cross-reaction was observed with serotype 2 antiserum and serotype 5 antigen. The results were reproducible and could be obtained in 4 h with only 10 microliters of serum for eight serotypes. Optimal antigen concentrations for the U. urealyticum serotypes ranged from 0.40 to 1.60 micrograms/ml. Our results indicated that enzyme-linked immunosorbent assay has the potential for the detection of antibodies to specific serotypes of U. urealyticum.
摘要
利用兔抗血清建立了用于检测解脲脲原体免疫球蛋白G抗体的微酶联免疫吸附测定系统的最佳条件。最初，针对在含马血清培养基上生长的8种解脲脲原体血清型产生的抗血清，与我们的酶联免疫吸附测定抗原呈现非特异性反应。用胎牛血清替代培养基中的马血清消除了这种非特异性。该测定法随后对最初的8种解脲脲原体血清型具有血清型特异性。明显的同源反应很容易与异源反应区分开来。观察到2型抗血清与5型抗原之间存在单向交叉反应。结果具有可重复性，仅用10微升血清针对8种血清型在4小时内即可获得结果。解脲脲原体血清型的最佳抗原浓度范围为0.40至1.60微克/毫升。我们的结果表明，酶联免疫吸附测定法有潜力用于检测解脲脲原体特定血清型的抗体。
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