通过顶空气相色谱法检测醇类和挥发性脂肪酸以鉴定厌氧菌。
Detection of alcohols and volatile fatty acids by head-space gas chromatography in identification of anaerobic bacteria.
作者信息
Larsson L, Märdh P A, Odham G
出版信息
J Clin Microbiol. 1978 Jan;7(1):23-7. doi: 10.1128/jcm.7.1.23-27.1978.
Abstract
A head-space gas chromatographic technique for the analysis of volatile bacterial metabolites is described. Bacteroides fragilis, Clostridium perfringens, and Propionibacterium acnes, cultured in a glucose-containing peptone yeast extract medium, were studied. The head-space technique was compared with the injection of the complete liquid culture medium, and solvent extracts thereof, into the gas chromatograph. Volatile fatty acids could be detected by all three methods, whereas alcohols produced by C. perfringens and P. acnes were detectable only in the head-space chromatograms. Both FFAP and Porapak Q were used as gas chromatography stationary phase. Porapak Q was found more suitable than FFAP for the separation of alcohols. The head-space technique requires a minimum of preparation before the analysis and is well suited to automation.
摘要
本文描述了一种用于分析挥发性细菌代谢产物的顶空气相色谱技术。研究了在含葡萄糖的蛋白胨酵母提取物培养基中培养的脆弱拟杆菌、产气荚膜梭菌和痤疮丙酸杆菌。将顶空技术与向气相色谱仪中注入完整的液体培养基及其溶剂提取物进行了比较。三种方法均能检测到挥发性脂肪酸，而产气荚膜梭菌和痤疮丙酸杆菌产生的醇类仅在顶空气相色谱图中可检测到。FFAP和Porapak Q均用作气相色谱固定相。发现Porapak Q比FFAP更适合分离醇类。顶空技术在分析前所需的准备工作最少，非常适合自动化操作。
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