Jass J R, England J, Miller K
J Clin Pathol. 1986 Apr;39(4):393-8. doi: 10.1136/jcp.39.4.393.
It has been suggested that diffuse changes in epithelial mucins may help to identify a subgroup of patients with ulcerative colitis who are at increased risk of developing colorectal cancer. Colonoscopic biopsy specimens were taken from 11 colitic patients shortly before colectomies were performed, in which cancer was identified. These patients were matched to colitic patients without cancer for age, sex, and duration of disease. Sections were stained with high iron diamine and alcian blue (HID-AB), mild periodic acid Schiff, and for peanut lectin binding sugar sequences. The sections were assessed blindly and graded semiquantitatively. Most showed ulcerative colitis in remission or mild active disease. There were no differences between the study and control groups. Metaplastic foci were more common in the group who had cancer. These showed increased staining of mucus by mild periodic acid Schiff and increased binding by peanut lectin to the supranuclear (Golgi) zone. Epithelial dysplasia (detected in four of the patients with cancer showed focal intense binding by peanut lectin to cytoplasm, mucus, and glycocalyx. Although dysplasia differed histochemically from normal tissue, special techniques did not facilitate its diagnosis.
有人提出，上皮粘蛋白的弥漫性变化可能有助于识别溃疡性结肠炎患者中患结直肠癌风险增加的一个亚组。在即将进行结肠切除术（术中发现癌症）之前，从11例结肠炎患者身上获取了结肠镜活检标本。这些患者在年龄、性别和病程方面与无癌症的结肠炎患者相匹配。切片用高铁二胺和阿尔辛蓝（HID-AB）、轻度过碘酸希夫染色，并检测花生凝集素结合糖序列。对切片进行盲法评估并进行半定量分级。大多数显示溃疡性结肠炎处于缓解期或轻度活动期。研究组和对照组之间没有差异。化生灶在患癌组中更常见。这些化生灶显示轻度过碘酸希夫染色的黏液增加，以及花生凝集素与核上（高尔基体）区的结合增加。上皮发育异常（在4例患癌患者中检测到）显示花生凝集素与细胞质、黏液和糖萼有局灶性强烈结合。尽管发育异常在组织化学上与正常组织不同，但特殊技术并未有助于其诊断。
