Díaz-Sánchez Luis M, Blanco-Tirado Cristian, Combariza Marianny Y
Escuela de Química, Universidad Industrial de Santander, Bucaramanga, Santander 680002, Colombia.
MethodsX. 2023 Mar 20;10:102140. doi: 10.1016/j.mex.2023.102140. eCollection 2023.
The method describes pigment analysis from microalgae/phytoplankton extracts using electron-transfer Matrix-Assisted Laser Desorption Ionization Mass Spectrometry (ET MALDI MS). Current microalgae/phytoplankton pigment analysis requires resource- and time-intensive chromatographic methods due to the broad polarity range of the target analytes. On the other hand, traditional MALDI MS chlorophyll analysis, using proton-transfer matrices such as 2,5-dihydroxybenzoic acid (DHB) or α-cyano-4-hydroxycinnamic acid (CHCA), results in central metal loss and phytol-ester cleavage. ET MALDI MS is an alternative for the rapid screening and detection of pigments in microalgae extracts.•MALDI matrices with ionization energies above 8.0 eV guarantee electron-transfer processes from photosynthetic and photoprotective pigments whose ionization energies lay below 7.5 eV.•ET MALDI MS pigment analysis agrees with data gathered from conventional chromatographic techniques (HPLC) and optical microscopy for pigment extracts from cultures and freshwater phytoplankton samples.•The ET MALDI MS method allows fast and reliable detection of pigments in microalgae cultures and freshwater phytoplankton samples
该方法描述了使用电子转移基质辅助激光解吸电离质谱（ET MALDI MS）对微藻/浮游植物提取物进行色素分析。由于目标分析物的极性范围较宽，目前的微藻/浮游植物色素分析需要耗费资源和时间的色谱方法。另一方面，使用质子转移基质（如2,5 - 二羟基苯甲酸（DHB）或α - 氰基 - 4 - 羟基肉桂酸（CHCA））的传统MALDI MS叶绿素分析会导致中心金属损失和植醇酯裂解。ET MALDI MS是快速筛选和检测微藻提取物中色素的一种替代方法。
•电离能高于8.0 eV的MALDI基质可确保来自电离能低于7.5 eV的光合色素和光保护色素的电子转移过程。
•ET MALDI MS色素分析与从传统色谱技术（HPLC）以及光学显微镜获得的关于培养物和淡水浮游植物样品色素提取物的数据一致。
•ET MALDI MS方法能够快速、可靠地检测微藻培养物和淡水浮游植物样品中的色素
