Kim Hee Kyung, Kim Hyun-Jo, Kim Jae Yun, Ban Myung Jin, Son Jiwon, Hwang Yongsung, Cho Sung Bin
Department of Pathology, Soonchunhyang University Bucheon Hospital, Bucheon, Korea.
CNP Skin Clinic, Cheonan, Korea.
Clin Cosmet Investig Dermatol. 2023 Mar 25;16:721-729. doi: 10.2147/CCID.S404631. eCollection 2023.
Single to multiple pulse packs of bipolar, alternating current radiofrequency (RF) oscillations have been used for various medical purposes using invasive microneedle electrodes. This study was designed to evaluate the effects of pulse widths and cycles of RF pulse packs on immediate and delayed thermal tissue reactions in in vivo rat skin.
RF energy at the frequency of 1 MHz and power of 70 W was delivered at each experimental setting into in vivo rat skin at 1.5-mm microneedle penetration, and then, tissue samples were obtained after 1 h and 3, 7, 14, and 21 days and histologically analyzed.
A single-pulse-pack RF treatment generated coagulative necrosis zones in the dermal peri-electrode area and zones of non-necrotic thermal reactions in the dermal inter-electrode area. Multiple pulse-pack, RF-treated rat skin specimens revealed that the number and size of peri-electrode coagulative necrosis were markedly decreased by increasing the number of pulse packs and accordingly decreasing the conduction time of each pulse pack. The microscopic changes in RF-induced non-necrotic thermal reaction in the inter-electrode area were more remarkable in specimens treated with RF of 7 or 10 pulse packs than in specimens treated with RF of 1-4 pulse packs.
The gated delivery of multiple RF pulse packs using a bipolar, alternating current, 1-MHz RF system using insulated microneedle electrodes efficiently generates non-necrotic thermal tissue reactions over the upper, mid, and deep dermis and subcutaneous fat in the inter-electrode areas.
单脉冲至多脉冲包的双极交流电射频（RF）振荡已通过侵入性微针电极用于各种医学目的。本研究旨在评估RF脉冲包的脉冲宽度和周期对大鼠活体皮肤即时和延迟热组织反应的影响。
在每个实验设置下，将频率为1 MHz、功率为70 W的RF能量通过1.5 mm的微针穿透传递到大鼠活体皮肤中，然后在1小时、3天、7天、14天和21天后获取组织样本并进行组织学分析。
单脉冲包RF治疗在真皮电极周围区域产生凝固性坏死区，在真皮电极间区域产生非坏死性热反应区。多脉冲包RF治疗的大鼠皮肤标本显示，通过增加脉冲包数量并相应减少每个脉冲包的传导时间，电极周围凝固性坏死的数量和大小显著减少。电极间区域RF诱导的非坏死性热反应的微观变化在接受7或10个脉冲包RF治疗的标本中比在接受1 - 4个脉冲包RF治疗的标本中更明显。
使用绝缘微针电极的双极1 MHz交流电RF系统进行多RF脉冲包的门控递送，可在电极间区域的上、中、深层真皮和皮下脂肪中有效产生非坏死性热组织反应。
