通过对核糖体ITS2和LSU区域进行泛真菌PCR/测序确诊的急性髓性白血病患者肝脾毛霉菌病：一例报告
Hepatosplenic mucormycosis due to identified by panfungal PCR/sequencing of ribosomal ITS2 and LSU regions in a patient with acute myelogenous leukemia: A case report.
作者信息
Gillrie Mark, Chow Barbara, Griener Thomas, Johnson Andrew, Church Deirdre
机构信息
Department of Medicine, Cumming School of Medicine, University of Calgary, Calgary, Alberta, Canada.
Department of Pathology & Laboratory Medicine, Cumming School of Medicine, University of Calgary, Calgary, Alberta, Canada.
出版信息
J Assoc Med Microbiol Infect Dis Can. 2023 Mar 1;8(1):105-110. doi: 10.3138/jammi-2022-0020. eCollection 2023 Mar.
BACKGROUND
Angioinvasive infection with dissemination to the liver and spleen is exceedingly uncommon, representing less than 1% of reported cases of mucormycosis.
METHODS
Diagnosis of mucormycosis is often difficult using conventional methods that rely on broad-based non-septate hyphae present on histologic examination and morphological identification of the cultured organism. Our laboratory also uses an in-house panfungal molecular assay to rapidly diagnose invasive fungal infection when conventional methods do not provide definitive results.
RESULTS
Herein we present a case of disseminated mucormycosis with hepatosplenic involvement in a 49-year-old female with acute myelogenous leukemia following induction chemotherapy. But in this case repeated tissue biopsy cultures were negative. infection was diagnosed using an in-house panfungal PCR/sequencing assay based on dual priming oligonucleotide primers.
CONCLUSIONS
New molecular assays facilitate prompt diagnosis of invasive fungal infections.
背景
血管侵袭性感染并播散至肝脏和脾脏极为罕见，占报告的毛霉病病例不到1%。
方法
使用依赖组织学检查中出现的宽基无隔菌丝和培养生物体形态鉴定的传统方法，毛霉病的诊断通常很困难。当传统方法不能提供明确结果时，我们实验室还使用内部泛真菌分子检测法快速诊断侵袭性真菌感染。
结果
在此，我们报告一例49岁急性髓性白血病女性在诱导化疗后发生的伴有肝脾受累的播散性毛霉病病例。但在该病例中，重复的组织活检培养均为阴性。基于双引物寡核苷酸引物的内部泛真菌PCR/测序检测法诊断出感染。
结论
新的分子检测法有助于及时诊断侵袭性真菌感染。
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