Zolkiflee Nur Farahani, Meor Mohd Affandi Meor Mohd Redzuan, Abdul Majeed Abu Bakar
Laboratory Fundamental of Pharmaceutics, Universiti Teknologi MARA (UiTM) Selangor, 42300, Bandar Puncak Alam, Selangor Darul Ehsan, Malaysia.
Pharmaceutical and Life Sciences ReNeU, Universiti Teknologi MARA (UiTM), 40450, Shah Alam, Selangor Darul Ehsan, Malaysia.
Heliyon. 2023 Mar 6;9(4):e14363. doi: 10.1016/j.heliyon.2023.e14363. eCollection 2023 Apr.
Levocetirizine dihydrochloride (LCTZ) is a second-generation antihistamine known for its efficacy without sedation. However, the binding mechanism with plasma protein which makes it devoid of sedative effects is yet to be uncovered. In this study, we elucidated thermodynamic parameters of solute-solvent and solute-cosolute interactions between LCTZ, and the amino acids l-alanine (Ala) and l-glutamine (Gln) in aqueous solutions. The volumetric and conductance parameters were calculated using the experimental density and conductance data of aqueous solutions of Ala and Gln (concentration range of 0.02-0.20 mol kg) and LCTZ (concentration range of 0.01, 0.07, and 0.13 mol kg) at 298.15, 303.15, 308.15, and 313.15 K. Volumetric analysis study revealed that the apparent molar volume (V), limiting apparent molar volume (V ), and Masson's coefficient (S) values indicated predominantly solute-solvent interactions that were influenced by the solute's concentration and temperature. Meanwhile, partial molar expansibilities (E ), transfer volume ΔV and Hepler's constant (δV/δT) established the solution system's structure-breaking tendency. The Gibb's free energy (ΔG) values derived from conductometry data indicated the system's spontaneity. These calculated constants provided a detailed understanding of the different types of intermolecular interactions found in the ternary (LCTZ + water + amino acids) system.
二盐酸左西替利嗪（LCTZ）是一种第二代抗组胺药，以其无镇静作用的疗效而闻名。然而，其与血浆蛋白的结合机制使其缺乏镇静作用，这一点尚未被揭示。在本研究中，我们阐明了LCTZ与氨基酸L-丙氨酸（Ala）和L-谷氨酰胺（Gln）在水溶液中的溶质-溶剂和溶质-共溶质相互作用的热力学参数。使用Ala和Gln（浓度范围为0.02 - 0.20 mol kg）以及LCTZ（浓度范围为0.01、0.07和0.13 mol kg）在298.15、303.15、308.15和313.15 K下的水溶液的实验密度和电导率数据计算了体积和电导率参数。体积分析研究表明，表观摩尔体积（V）、极限表观摩尔体积（V ）和马森系数（S）值主要表明溶质-溶剂相互作用受溶质浓度和温度的影响。同时，偏摩尔膨胀系数（E ）、转移体积ΔV 和赫普勒常数（δV/δT）确定了溶液体系的结构破坏趋势。由电导率数据得出的吉布斯自由能（ΔG）值表明了体系的自发性。这些计算出的常数详细地揭示了三元（LCTZ + 水 + 氨基酸）体系中存在的不同类型的分子间相互作用。
