非整合型质粒折叠染色体复合物的生化特性：性因子F与大肠杆菌类核
Biochemical characterization of nonintegrated plasmid-folded chromosome complexes: sex factor F and the Escherichia coli nucleoid.
作者信息
Miller J R, Kline B C
出版信息
J Bacteriol. 1979 Feb;137(2):885-90. doi: 10.1128/jb.137.2.885-890.1979.
Abstract
The existence of nonintegrated plasmid-chromosome complexes has been deduced in previous work from the cosedimentation of covalently closed, circular plasmids with host folded chromosomes. In the present work, it is shown that about 70 to 90% of the covalently closed, circular F deoxyribonucleic acid could be released in vitro from chromosome complexes by ribonuclease treatment but not by protease, Sarkosyl, or ethidium bromide. Consistent with the in vitro studies, Escherichia coli cells treated for 5 min with rifampin, an inhibitor of ribonucleic acid initiation, released upon lysis 90% of their plasmid deoxyribonucleic acid as freely sedimenting molecules.
摘要
在之前的研究工作中，通过共价闭合环状质粒与宿主折叠染色体的共沉降现象，推测出了非整合型质粒 - 染色体复合物的存在。在本研究中发现，约70%至90%的共价闭合环状F脱氧核糖核酸可在体外通过核糖核酸酶处理从染色体复合物中释放出来，但蛋白酶、十二烷基肌氨酸钠或溴化乙锭处理则无法实现。与体外研究结果一致，用核糖核酸起始抑制剂利福平处理5分钟的大肠杆菌细胞，在裂解后可释放出90%的质粒脱氧核糖核酸，这些分子沉降自由。
相似文献
1
Biochemical characterization of nonintegrated plasmid-folded chromosome complexes: sex factor F and the Escherichia coli nucleoid.非整合型质粒折叠染色体复合物的生化特性：性因子F与大肠杆菌类核
J Bacteriol. 1979 Feb;137(2):885-90. doi: 10.1128/jb.137.2.885-890.1979.
2
Partial characterization of nucleoids and nucleoid-plasmid complexes from Salmonella typhimurium.鼠伤寒沙门氏菌类核及类核-质粒复合物的部分特性分析
J Bacteriol. 1976 Jul;127(1):664-6. doi: 10.1128/jb.127.1.664-666.1976.
3
Detection of nonintegrated plasmid deoxyribonucleic acid in the folded chromosome of Escherichia coli: physiochemical approach to studying the unit of segregation.在大肠杆菌折叠染色体中检测非整合质粒脱氧核糖核酸：研究分离单位的物理化学方法
J Bacteriol. 1975 Jan;121(1):165-72. doi: 10.1128/jb.121.1.165-172.1975.
4
Method for obtaining more-accurate covalently closed circular plasmid-to-chromosome ratios from bacterial lysates by dye-buoyant density centrifugation.通过染料浮力密度离心法从细菌裂解物中获得更准确的共价闭合环状质粒与染色体比率的方法。
J Bacteriol. 1977 Apr;130(1):148-53. doi: 10.1128/jb.130.1.148-153.1977.
5
Nonintegrated plasmid-chromosome complexes in Escherichia coli.大肠杆菌中的非整合质粒 - 染色体复合体
J Bacteriol. 1976 Aug;127(2):881-9. doi: 10.1128/jb.127.2.881-889.1976.
6
Comparative studies on membrane-associated, folded chromosomes from Escherichia coli.大肠杆菌膜相关折叠染色体的比较研究。
J Bacteriol. 1976 Apr;126(1):64-71. doi: 10.1128/jb.126.1.64-71.1976.
7
Relationship of R6K replicating forms to the folded chromosome of Escherichia coli.R6K复制形式与大肠杆菌折叠染色体的关系。
J Bacteriol. 1978 Aug;135(2):476-82. doi: 10.1128/jb.135.2.476-482.1978.
8
A mild method for the isolation of folded chromosomes from Escherichia coli.
Z Allg Mikrobiol. 1975;15(4):231-41. doi: 10.1002/jobm.3630150403.
9
Unfolding of the chromosome of Escherichia coli after treatment with rifampicin.
Z Allg Mikrobiol. 1975;15(4):243-7. doi: 10.1002/jobm.3630150404.
10
F deoxyribonucleic acid transferred to recipient cells in the presence of rifampin.在利福平存在的情况下转移至受体细胞的F脱氧核糖核酸。
J Bacteriol. 1975 Mar;121(3):1000-6. doi: 10.1128/jb.121.3.1000-1006.1975.
引用本文的文献
1
Bacterial nucleoid is a riddle wrapped in a mystery inside an enigma.细菌拟核是一个谜团，被包裹在一个谜中，又被包含在另一个谜里。
J Bacteriol. 2024 Mar 21;206(3):e0021123. doi: 10.1128/jb.00211-23. Epub 2024 Feb 15.
2
Cellular location of Mu DNA replicas.缪氏DNA复制体的细胞定位
J Virol. 1981 Apr;38(1):249-55. doi: 10.1128/JVI.38.1.249-255.1981.
3
Cloning of E. coli pnp gene from an episome.从附加体克隆大肠杆菌多核苷酸磷酸化酶基因。
Mol Gen Genet. 1981;183(2):298-305. doi: 10.1007/BF00270632.
4
Construction and use of pyr::lac fusion strains to study regulation of pyrimidine biosynthesis in Salmonella typhimurium.构建和使用pyr::lac融合菌株以研究鼠伤寒沙门氏菌中嘧啶生物合成的调控。
Mol Gen Genet. 1983;189(3):463-70. doi: 10.1007/BF00325910.
本文引用的文献
1
A dye-buoyant-density method for the detection and isolation of closed circular duplex DNA: the closed circular DNA in HeLa cells.一种用于检测和分离闭环双链DNA的染料浮力密度法：HeLa细胞中的闭环DNA
Proc Natl Acad Sci U S A. 1967 May;57(5):1514-21. doi: 10.1073/pnas.57.5.1514.
2
F 1 sex factor of Escherichia coli. Size and purification in the form of a strand-specific relaxation complex of supercoiled deoxyribonucleic acid and protein.大肠杆菌的F1性因子。以超螺旋脱氧核糖核酸与蛋白质的链特异性松弛复合体形式存在的大小及纯化方法。
Biochemistry. 1971 Dec 21;10(26):4975-80. doi: 10.1021/bi00802a022.
3
The folded genome of Escherichia coli isolated in a protein-DNA-RNA complex.在蛋白质 - DNA - RNA 复合物中分离出的大肠杆菌折叠基因组。
Proc Natl Acad Sci U S A. 1971 Jan;68(1):6-9. doi: 10.1073/pnas.68.1.6.
4
RNA molecules bound to the folded bacterial genome stabilize DNA folds and segregate domains of supercoiling.与折叠的细菌基因组结合的RNA分子可稳定DNA折叠并分离超螺旋结构域。
Cold Spring Harb Symp Quant Biol. 1974;38:31-41. doi: 10.1101/sqb.1974.038.01.006.
5
Properties of a membrane-attached form of the folded chromosome of Escherichia coli.大肠杆菌折叠染色体膜附着形式的特性
J Mol Biol. 1974 Jan 5;82(1):91-105. doi: 10.1016/0022-2836(74)90576-2.
6
Membrane attachment of R-factor deoxyribonucleic acid in compatible and incompatible cell pairs following conjugation.接合后R因子脱氧核糖核酸在相容和不相容细胞对中的膜附着情况。
J Bacteriol. 1973 Sep;115(3):1208-11. doi: 10.1128/jb.115.3.1208-1211.1973.
7
On the structure of the folded chromosome of Escherichia coli.关于大肠杆菌折叠染色体的结构
J Mol Biol. 1972 Nov 14;71(2):127-47. doi: 10.1016/0022-2836(72)90342-7.
8
Independence of F replication and chromosome replication in Escherichia coli.大肠杆菌中F因子复制与染色体复制的独立性
Mol Gen Genet. 1975;138(2):143-55. doi: 10.1007/BF02428118.
9
Conformational transitions in the Escherichia coli chromosome: analysis by viscometry and sedimentation.大肠杆菌染色体中的构象转变：通过粘度测定法和沉降法进行分析。
J Mol Biol. 1975 Oct 25;98(2):393-411. doi: 10.1016/s0022-2836(75)80126-4.
10
Detection of nonintegrated plasmid deoxyribonucleic acid in the folded chromosome of Escherichia coli: physiochemical approach to studying the unit of segregation.在大肠杆菌折叠染色体中检测非整合质粒脱氧核糖核酸：研究分离单位的物理化学方法
J Bacteriol. 1975 Jan;121(1):165-72. doi: 10.1128/jb.121.1.165-172.1975.
Zotero 插件