Michaels G, Kelln R A
Mol Gen Genet. 1983;189(3):463-70. doi: 10.1007/BF00325910.
The technique developed by Rosenfeld and Brenchley [J Bacteriol 144, 848-851 (1980)] has been used to introduce Mu d1 (Apr lac) into Salmonella typhimurium for purposes of constructing pyr::lac fusion strains. A stable pyrB::lac fusion mutant was subsequently derived and used for the genetic characterization of the pyrB gene. The direction of transcription of pyrB was determined to be counterclockwise on the S. typhimurium linkage map and argI was shown to be located clockwise of pyrB. Mutants altered in the regulation of expression of pyrB were isolated and two of the isolates chosen for further study were tentatively categorized as promoter or operator mutants.
罗森菲尔德和布伦奇利[《细菌学杂志》144, 848 - 851(1980)]开发的技术已被用于将Mu d1(Apr lac)导入鼠伤寒沙门氏菌，以构建pyr::lac融合菌株。随后获得了一个稳定的pyrB::lac融合突变体，并用于pyrB基因的遗传特征分析。确定pyrB的转录方向在鼠伤寒沙门氏菌连锁图谱上是逆时针的，并且argI位于pyrB的顺时针方向。分离出了在pyrB表达调控方面发生改变的突变体，所选择的两个用于进一步研究的分离株被初步归类为启动子或操纵子突变体。
