大肠杆菌膜相关折叠染色体的比较研究。
Comparative studies on membrane-associated, folded chromosomes from Escherichia coli.
作者信息
Dworsky P
出版信息
J Bacteriol. 1976 Apr;126(1):64-71. doi: 10.1128/jb.126.1.64-71.1976.
Abstract
Nuclear bodies were isolated from Escherichia coli spheroplasts by two different lysis procedures. Their lipid and protein content and the superhelix density was measured. The preparations differed mainly in respect to the amount of the attached membrane material, which seems to be an essential factor in maintaining the stability of the nuclear bodies. The superhelix density was found to be higher than that of naturally occurring, covalently closed, circular deoxyribonucleic acids. The influence of the preparative method on the superhelicity was comparatively small.
摘要
通过两种不同的裂解程序从大肠杆菌原生质球中分离出核小体。测量了它们的脂质和蛋白质含量以及超螺旋密度。这些制剂的主要差异在于附着膜材料的量，这似乎是维持核小体稳定性的一个重要因素。发现超螺旋密度高于天然存在的共价闭合环状脱氧核糖核酸的超螺旋密度。制备方法对超螺旋性的影响相对较小。
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