Avcilar-Kucukgoze Irem, Kashina Anna S
Department of Biomedical Sciences, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, PA, USA.
Department of Animal Biology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, PA, USA.
Methods Mol Biol. 2023;2620:93-99. doi: 10.1007/978-1-0716-2942-0_12.
This chapter describes the preparation of tRNA by in vitro transcription. tRNA produced by this method can be efficiently utilized for in vitro arginylation assays, following aminoacylation with Arg-tRNA synthetase, either directly during the arginylation reaction or separately to produce the purified preparation of Arg-tRNA. tRNA charging is described in other chapters of this book.
本章介绍了通过体外转录制备tRNA的方法。用这种方法产生的tRNA在与精氨酸tRNA合成酶进行氨酰化反应后，可直接用于体外精氨酸化测定，即在精氨酸化反应过程中使用，或单独用于制备纯化的精氨酸tRNA。本书的其他章节介绍了tRNA的氨酰化。
