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氨苄青霉素抗性转座子Tn1701在一组小型非转移性质粒中的定位。

Location of an ampicillin resistance transposon, Tn1701, in a group of small, nontransferring plasmids.

作者信息

Yamada Y, Calame K L, Grindley J N, Nakada D

出版信息

J Bacteriol. 1979 Feb;137(2):990-9. doi: 10.1128/jb.137.2.990-999.1979.

Abstract

By restriction endonuclease cleavage mapping and electron microscopic examination of heteroduplexes, we have identified an ampicillin resistance determinant transposon, designated Tn1701, in a group of small, nontransferring plasmids which confer resistance to ampicillin (Ap), sulfonamide (Su), and streptomycin (Sm). Plasmid NTP1, which mediates Ap resistance, contains Tn1701. Recombinant plasmids NTP3 (Ap Su) and NTP4 (Ap Su Sm) contain Tn1701, indicating that they were derived by transposition of Tn1701 from NTP1 to an unrelated plasmid, NTP2 (Su Sm). The transposon Tn1701 is very similar to the known ampicillin resistance transposons Tn1, Tn2, and Tn3 in its size (3.2 x 10(6) daltons), base sequence homology observed by heteroduplex formation, restriction endonuclease cleavage sites, and possession of a short inverted repeat sequence at both ends. Like the other TnA elements, Tn1701 also specifies a type TEM beta-lactamase.

摘要

通过限制性内切酶切割图谱分析以及异源双链体的电子显微镜检查,我们在一组赋予氨苄青霉素(Ap)、磺胺类药物(Su)和链霉素(Sm)抗性的小型非转移性质粒中,鉴定出了一个氨苄青霉素抗性决定因子转座子,命名为Tn1701。介导Ap抗性的质粒NTP1含有Tn1701。重组质粒NTP3(Ap Su)和NTP4(Ap Su Sm)也含有Tn1701,这表明它们是通过Tn1701从NTP1转座到一个不相关的质粒NTP2(Su Sm)而产生的。转座子Tn1701在大小(3.2×10⁶道尔顿)、通过异源双链体形成观察到的碱基序列同源性、限制性内切酶切割位点以及两端具有短反向重复序列等方面,与已知的氨苄青霉素抗性转座子Tn1、Tn2和Tn3非常相似。与其他TnA元件一样,Tn1701也编码一种TEM型β-内酰胺酶。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4329/218384/b51ff90bc119/jbacter00285-0299-a.jpg

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