Key Laboratory of Optic-electric Sensing and Analytical Chemistry for Life Science, Ministry of Education, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao, 266042, China.
Key Laboratory of Optic-electric Sensing and Analytical Chemistry for Life Science, Ministry of Education, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao, 266042, China; Institute for Frontier Materials, Deakin University, Geelong Waurn Ponds Campus, Geelong, Victoria, 3216, Australia.
Biosens Bioelectron. 2023 Jun 15;230:115287. doi: 10.1016/j.bios.2023.115287. Epub 2023 Mar 31.
To develop highly accurate and ultrasensitive strategies is of great importance for the clinical measurement, in particular, the detection of cancer biomarkers. Herein, we synthesized an ultrasensitive TiO/MXene/CdS QDs (TiO/MX/CdS) heterostructure as a photoelectrochemical immunosensor, which favors energy levels matching and fast electron transfer from CdS to TiO in the help of ultrathin MXene nanosheet. Dramatic photocurrent quenching can be observed upon incubation of the TiO/MX/CdS electrode by Cu solution from 96-well microplate, which caused by the formation of CuS and subsequent CuS (x = 1, 2), reducing the absorption of light and boosting the electron-hole recombination upon irradiation. As a result, the as-prepared biosensor demonstrates a linearly increased photocurrent quenching percentage (Q%) value with CEA concentration ranging from 1 fg/mL to 10 ng/mL, as well as a low detection limit of 0.24 fg/mL. Benefit from its excellent stability, high selectivity and good reproducibility of as-prepared PEC immunosensor, we believe that this proposed strategy might provide new opportunities for clinical diagnosis of CEA and other tumor markers.
为了进行临床测量，尤其是癌症生物标志物的检测，开发高度准确和超灵敏的策略非常重要。在此，我们合成了一种超灵敏的 TiO/MXene/CdS QDs（TiO/MX/CdS）异质结构作为光电化学免疫传感器，得益于超薄 MXene 纳米片，有利于能级匹配和 CdS 向 TiO 的快速电子转移。当将 TiO/MX/CdS 电极用 Cu 溶液孵育时，从 96 孔微孔板中可以观察到明显的光电流猝灭，这是由于形成了 CuS 和随后的 CuS（x=1，2），从而减少了光的吸收，并在照射时促进了电子-空穴复合。结果，所制备的生物传感器在 CEA 浓度范围为 1 fg/mL 至 10 ng/mL 时表现出光电流猝灭百分比（Q%）值线性增加，检测限低至 0.24 fg/mL。得益于所制备的 PEC 免疫传感器的出色稳定性、高选择性和良好的重现性，我们相信这种策略可能为 CEA 和其他肿瘤标志物的临床诊断提供新的机会。
