Institute of Entomology, Guizhou University, Guiyang 550025, China.
Guizhou Provincial Key Laboratory for Agricultural Pest Management of the Mountainous Region, Guiyang 550025, China.
Genes (Basel). 2023 Feb 4;14(2):414. doi: 10.3390/genes14020414.
In this study, the mitochondrial genome of was assembled and annotated, which is a circular DNA molecule including 13 protein-coding genes (PCGs), 22 transfer RNA (tRNA) genes, 2 ribosomal RNA genes (12S rRNA and 16S rRNA), and 1388 bp non-coding regions (A + T rich region), measuring 16,021 bp in length. The nucleotide composition of the mitochondrial genome is 41.3% adenine (A), 38.7% thymine (T), 8.4% guanine (G), and 11.6% cytosine (C). Most of the protein-coding genes presented a typical ATN start codon (ATA, ATT, ATC, ATG), except for ND1, which showed the start codon TTG. Three-quarters of the protein-coding genes showed the complete stop codon TAR (TAA, TAG), except the genes COI, COII, ND4, and ND5, which showed incomplete stop codons (T- or TA-). All the tRNA genes have the typical clover-leaf structure, except tRNA (AGN), which has a missing dihydrouridine arm (DHU). The phylogenetic results determined by both maximum likelihood and Bayesian inference methods consistently supported the monophyly of the subfamily Galerucinae and revealed that the subtribe Luperina and genus are polyphyletic groups. Meanwhile, the classification status of the genus is controversial.
在这项研究中，组装并注释了 的线粒体基因组，这是一个 circular DNA 分子，包括 13 个蛋白编码基因（PCGs）、22 个转移 RNA（tRNA）基因、2 个核糖体 RNA 基因（12S rRNA 和 16S rRNA）和 1388bp 的非编码区（富含 A + T 区），全长 16021bp。线粒体基因组的核苷酸组成是 41.3%的腺嘌呤（A）、38.7%的胸腺嘧啶（T）、8.4%的鸟嘌呤（G）和 11.6%的胞嘧啶（C）。大多数蛋白编码基因呈现典型的 ATN 起始密码子（ATA、ATT、ATC、ATG），除了 ND1，它显示的起始密码子是 TTG。四分之三的蛋白编码基因显示完整的终止密码子 TAR（TAA、TAG），除了 COI、COII、ND4 和 ND5 基因，它们显示不完整的终止密码子（T- 或 TA-）。所有的 tRNA 基因都具有典型的三叶草结构，除了 tRNA（AGN），它缺少二氢尿嘧啶臂（DHU）。最大似然法和贝叶斯推断法的系统发育结果都一致支持了叶甲亚科的单系性，并揭示了 Luperina 亚族和属 是多系群。同时，属 的分类地位存在争议。
