A Wearable Fiber-Free Optical Sensor for Continuous Monitoring of Cerebral Blood Flow in Freely Behaving Mice.

OBJECTIVE

Wearable technologies for functional brain monitoring in freely behaving subjects can advance our understanding of cognitive processing and adaptive behavior. Existing technologies are lacking in this capability or need procedures that are invasive and/or otherwise impede brain assessments during social behavioral conditions, exercise, and sleep.

METHODS

In response a complete system was developed to combine relative cerebral blood flow (rCBF) measurement, O and CO supplies, and behavior recording for use on conscious, freely behaving mice. An innovative diffuse speckle contrast flowmetry (DSCF) device and associated hardware were miniaturized and optimized for rCBF measurements in small subject applications. The use of this wearable, fiber-free, near-infrared DSCF head-stage/probe allowed no craniotomy, minimally invasive probe implantation, and minimal restraint of the awake animal.

RESULTS AND CONCLUSIONS

Significant correlations were found between measurements with the new DSCF design and an optical standard. The system successfully detected rCBF responses to CO-induced hypercapnia in both anesthetized and freely behaving mice.

SIGNIFICANCE

Collecting rCBF and activity information together during natural behaviors provides realistic physiological results and opens the path to exploring their correlations with pathophysiological conditions.