IEEE Trans Nanobioscience. 2023 Jul;22(3):655-663. doi: 10.1109/TNB.2022.3233378. Epub 2023 Jun 29.
In recent years, nanoparticles camouflaged by red blood cell membrane (RBCM) have become a potential nano-drug delivery platform due to their good biocompatibility and immune evasion capability. Here, a multifunctional drug nanocarrier based on RBCM camouflaged mesoporous silica nanorods (MSNR) is presented, which can be used in pH and near-infrared (NIR) light triggered synergistic chemo-photothermal killing of cancer cells. To fabricate such a nanocarrier, MSNR and RBCM were prepared by the sol-gel method and modified hypotonic lysis method, respectively. Drugs were loaded into the pores of MSNR. Finally, RBCM was coated on the surface of MSNR by extrusion through a polycarbonate membrane. The advantages of the nanocarrier include: 1) MSNR can induce more cellular uptake than sphere shaped mesoporous silica nanoparticles. 2) The RBCM can reduce drug leakage and prevent clearance of the nanocarriers by macrophages. 3) By simultaneous loading doxorubicin (DOX) and indocyanine green (ICG), pH and NIR triggered synergistic chemo-photothermal therapy can be realized. In the experiment, we studied the drug releasing and cellular uptake of the nanocarriers in a breast cancer cell line (SKBR3 cells), in which a sufficient killing effect was observed. Such a multifunctional drug nanocarrier holds a broad application prospect in cancer treatment.
近年来，由于具有良好的生物相容性和免疫逃逸能力，被红细胞膜（RBCM）伪装的纳米颗粒成为一种有潜力的纳米药物递送平台。在这里，提出了一种基于 RBCM 伪装介孔硅纳米棒（MSNR）的多功能药物纳米载体，可用于 pH 和近红外（NIR）光触发的协同化学-光热杀伤癌细胞。为了制备这种纳米载体，分别通过溶胶-凝胶法和改良的低渗裂解法制备 MSNR 和 RBCM。将药物载入 MSNR 的孔中。最后，通过聚碳酸酯膜挤出将 RBCM 涂覆在 MSNR 的表面上。该纳米载体的优点包括：1）MSNR 可诱导比球形介孔硅纳米颗粒更多的细胞摄取。2）RBCM 可减少药物泄漏并防止巨噬细胞清除纳米载体。3）通过同时装载阿霉素（DOX）和吲哚菁绿（ICG），可实现 pH 和 NIR 触发的协同化学-光热治疗。在实验中，我们研究了纳米载体在乳腺癌细胞系（SKBR3 细胞）中的药物释放和细胞摄取情况，观察到了足够的杀伤效果。这种多功能药物纳米载体在癌症治疗中有广阔的应用前景。
