Department of Botany, Government College Women University, Faisalabad, Pakistan.
Department of Botany, University of Agriculture, Faisalabad, Pakistan.
Plant Signal Behav. 2023 Dec 31;18(1):2186045. doi: 10.1080/15592324.2023.2186045.
Drought stress poorly impacts many morphological and physio-biochemical processes in plants. Pea ( L.) plants are highly nutritious crops destined for human consumption; however, their productivity is threatened under drought stress. Thiamine (vitamin B1) is well-known essential micronutrient, acting as a cofactor in key metabolic processes. Therefore, this study was designed to examine the protective effect of foliar application of thiamine (0, 250, and 500 ppm) on two varieties of pea plants under drought stress. Here, we conducted the pot experiment at the Government College Women University, Faisalabad, to investigate the physio-biochemical and morphological traits of two pea varieties (sarsabz and metior) grown under drought stress and thiamine treatment. Drought stress was applied to plants after germination period of 1 month. Results showed that root fresh and dry weight, shoot fresh and dry weight, number of pods, leaf area, total soluble sugars, total phenolics, total protein contents, catalase, peroxidase, and mineral ions were reduced against drought stress. However, the application of thiamine (both 250 and 500 ppm) overcome the stress and also enhances these parameters, and significantly increases the antioxidant activities (catalase and peroxidase). Moreover, the performance of sarsabz was better under control and drought stress conditions than metior variety. In conclusion, the exogenous application of thiamine enabled the plants to withstand drought stress conditions by regulating several physiological and biochemical mechanisms. In agriculture, it is a great latent to alleviate the antagonistic impact of drought stress on crops through the foliar application of thiamine.
干旱胁迫严重影响植物的许多形态和生理生化过程。豌豆（L.）是一种高营养的作物，供人类食用；然而，它们的生产力受到干旱胁迫的威胁。硫胺素（维生素 B1）是一种众所周知的必需微量营养素，作为关键代谢过程的辅助因子。因此，本研究旨在研究叶面喷施硫胺素（0、250 和 500ppm）对两种豌豆品种在干旱胁迫下的保护作用。在这里，我们在费萨拉巴德的女子政府学院进行了盆栽实验，以研究两种豌豆品种（sarsabz 和 metior）在干旱胁迫和硫胺素处理下的生理生化和形态特征。干旱胁迫在植物发芽后 1 个月开始施加。结果表明，根鲜重和干重、茎鲜重和干重、豆荚数、叶面积、总可溶性糖、总酚、总蛋白含量、过氧化氢酶、过氧化物酶和矿物质离子对干旱胁迫的抵抗力下降。然而，硫胺素（250 和 500ppm）的应用克服了应激，也提高了这些参数，并显著提高了抗氧化活性（过氧化氢酶和过氧化物酶）。此外，sarsabz 在对照和干旱胁迫条件下的表现优于 metior 品种。总之，外源硫胺素通过调节几种生理生化机制，使植物能够耐受干旱胁迫条件。在农业中，通过叶面喷施硫胺素来缓解干旱胁迫对作物的拮抗影响是一种很有潜力的方法。
