Department of Anatomy, Histology & Developmental Biology, Medical School, Shenzhen University, Shenzhen, People's Republic of China.
Department of Trauma and Orthopedics, The Affiliated Baoan Hospital of Southern Medical University, Shenzhen, People's Republic of China.
Stem Cells Transl Med. 2023 May 15;12(5):245-257. doi: 10.1093/stcltm/szad019.
Severe trauma or chronic wounds can deplete the keratinocyte stem cells (KSCs) present in the epidermal basal layer or inhibit their migration leading to compromised wound healing. Supplementing KSCs is the key to solution while lineage reprogramming provides a new approach to acquiring KSCs. Through direct lineage reprogramming, induced KSCs (iKSCs) can be produced from somatic cells, which exhibit great application potential. Two strategies are currently being used to directly generate iKSCs, lineage transcription factor (TF)-mediated and pluripotency factors-mediated. This review focuses on lineage TF-mediated direct reprogramming and describes the conversion process along with the underlying epigenetic mechanisms. It also discusses other potential induction strategies to generate iKSCs and challenges associated with in situ reprogramming for skin repair.
严重创伤或慢性伤口会耗尽表皮基底层中存在的角质细胞干细胞 (KSCs) 或抑制其迁移，从而导致伤口愈合受损。补充 KSCs 是解决问题的关键，而谱系重编程为获得 KSCs 提供了一种新方法。通过直接谱系重编程，可以从体细胞中产生诱导的角质细胞干细胞 (iKSCs)，具有巨大的应用潜力。目前有两种策略可用于直接生成 iKSCs，即谱系转录因子 (TF) 介导和多能性因子介导。本文重点介绍了 TF 介导的直接重编程，并描述了转化过程以及潜在的表观遗传机制。它还讨论了其他潜在的诱导策略来产生 iKSCs 以及与皮肤修复原位重编程相关的挑战。
