探索乙醇引起群体运动活性变化的电路基础的方法。
Methods for Exploring the Circuit Basis of Ethanol-Induced Changes in Group Locomotor Activity.
机构信息
Molecular Pharmacology and Physiology Graduate Program, Brown University, Providence, Rhode Island 02912, USA.
Department of Psychology, Bryant University, Smithfield, Rhode Island 02917, USA.
出版信息
Cold Spring Harb Protoc. 2023 Oct 3;2023(10):108138. doi: 10.1101/pdb.prot108138.
Locomotion is a behavioral readout that can be used to understand responses to specific stimuli or perturbations. The fly Group Activity Monitor (flyGrAM) provides a high-throughput and high-content readout of the acute stimulatory and sedative effects of ethanol. The flyGrAM system is adaptable and seamlessly introduces thermogenetic or optogenetic stimulation to dissect neural circuits underlying behavior and tests responses to other volatilized stimuli (humidified air, odorants, anesthetics, vaporized drugs of abuse, etc.). The automated quantification and readout of activity provide users with a real-time representation of the group activity within each chamber throughout the experiment, helping users to quickly determine proper ethanol doses and duration, run behavioral screens, and plan follow-up experiments.
运动是一种行为读数，可以用来了解对特定刺激或干扰的反应。飞行群活动监测器（flyGrAM）提供了高通量和高内涵的急性刺激和镇静作用乙醇读数。flyGrAM 系统具有适应性，可以无缝引入热敏或光遗传学刺激，以剖析行为背后的神经回路，并测试对其他挥发刺激（加湿空气、气味剂、麻醉剂、挥发滥用药物等）的反应。活动的自动量化和读数为用户提供了每个室内组活动的实时表示，帮助用户快速确定适当的乙醇剂量和持续时间，运行行为筛选，并计划后续实验。
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