Molecular Pharmacology and Physiology Graduate Program, Brown University, Providence, Rhode Island 02912, USA.
Department of Psychology, Bryant University, Smithfield, Rhode Island 02917, USA.
Cold Spring Harb Protoc. 2023 Oct 3;2023(10):719-24. doi: 10.1101/pdb.top107887.
is a powerful genetic model for investigating the mechanisms underlying ethanol-induced behaviors, metabolism, and preference. Ethanol-induced locomotor activity is especially useful for understanding the mechanisms by which ethanol acutely affects the brain and behavior. Ethanol-induced locomotor activity is characterized by hyperlocomotion and subsequent sedation with increased exposure duration or concentration. Locomotor activity is an efficient, easy, robust, and reproducible behavioral screening tool for identifying underlying genes and neuronal circuits as well as investigating genetic and molecular pathways. We introduce a detailed protocol for performing experiments investigating how volatilized ethanol affects locomotor activity using the fly Group Activity Monitor (flyGrAM). We introduce installation, implementation, data collection, and subsequent data-analysis methods for investigating how volatilized stimuli affect activity. We also introduce a procedure for how to optogenetically probe neuronal activity to identify the neural mechanisms underlying locomotor activity.
是一种强大的遗传模型，可用于研究乙醇诱导的行为、代谢和偏好的机制。乙醇诱导的运动活性特别有助于了解乙醇如何急性影响大脑和行为。乙醇诱导的运动活性表现为超运动和随后的镇静，随着暴露持续时间或浓度的增加。运动活性是一种有效的、简单的、稳健的和可重复的行为筛选工具，用于鉴定潜在的基因和神经元回路，以及研究遗传和分子途径。我们介绍了一种详细的方案，用于使用飞行动物组活动监测器（flyGrAM）进行实验，研究挥发乙醇如何影响运动活性。我们介绍了安装、实施、数据收集以及随后的数据分析方法，用于研究挥发刺激物如何影响活性。我们还介绍了一种光遗传探测神经元活动的方法，以确定运动活性的神经机制。
