Wang Jing, Shen Yiyu, Wang Xiaoguang, Zhou Zhongcheng, Zhong Zhengxiang, Gu Tianyuan, Wu Bin
Department of Hepatobiliary Surgery, The Second Affiliated Hospital of Jiaxing University, Jiaxing 314000, Zhejiang Province, China.
Department of Hepatobiliary Surgery, The Second Affiliated Hospital of Jiaxing University, No. 397, Huancheng North Road, Jiaxing 314000, Zhejiang Province, China.
Open Med (Wars). 2023 Apr 1;18(1):20230661. doi: 10.1515/med-2023-0661. eCollection 2023.
Our pre-investigation has revealed that long non-coding RNA (LncRNA) AL137789.1 has the potential to predict the survival of patients with pancreatic carcinoma (PCa). Accordingly, the mechanism underlying the implication of AL137789.1 in PCa is covered in the current study. The non-tumor and paired tumor tissues were collected. Kaplan-Meier curve was employed to estimate the survival of PCa patients with high or low expression of AL137789.1. The proliferation, migration, invasion, and cell cycle of PCa cells were determined, and the cytotoxicity of CD8 T cells was evaluated as well. Levels of AL137789.1, E-cadherin, N-cadherin, and Vimentin were quantified. According to the experimental results, AL137789.1 was highly expressed in PCa and related to a poor prognosis of patients. Overexpressed AL137789.1 enhanced the proliferation, migration, and invasion of PCa cells, increased the cell population at G2/M and S phases yet decreased that in G0/G1 phase, and diminished the cytotoxicity of CD8 T cells. Also, overexpressed AL137789.1 elevated levels of N-cadherin and Vimentin, while lessening E-cadherin levels. However, the silencing of AL137789.1 produced contrary effects. Collectively, lncRNA AL137789.1 plays a tumor-promotive role in PCa by enhancing the progression and immune escape.
我们的前期研究表明，长链非编码RNA（LncRNA）AL137789.1具有预测胰腺癌（PCa）患者生存的潜力。因此，本研究探讨了AL137789.1在PCa中的作用机制。收集非肿瘤组织和配对的肿瘤组织。采用Kaplan-Meier曲线评估AL137789.1高表达或低表达的PCa患者的生存率。检测PCa细胞的增殖、迁移、侵袭和细胞周期，并评估CD8 T细胞的细胞毒性。定量检测AL137789.1、E-钙黏蛋白、N-钙黏蛋白和波形蛋白的水平。实验结果显示，AL137789.1在PCa中高表达，且与患者预后不良相关。过表达AL137789.1可增强PCa细胞的增殖、迁移和侵袭能力，增加G2/M期和S期细胞比例，降低G0/G1期细胞比例，并降低CD8 T细胞的细胞毒性。此外，过表达AL137789.1可提高N-钙黏蛋白和波形蛋白水平，降低E-钙黏蛋白水平。然而，沉默AL137789.1则产生相反的效果。综上所述，lncRNA AL137789.1通过促进肿瘤进展和免疫逃逸在PCa中发挥促癌作用。
