Maggi C A, Santicioli P, Meli A
J Pharmacol Methods. 1986 Apr;15(2):157-67. doi: 10.1016/0160-5402(86)90064-1.
A new cystometric procedure that involves a nonstop transvesical (through a needle inserted into the bladder dome) infusion of saline in urethane-anesthetized rats is described. This permits the obtainment of a series of repetitive voiding cycles in both male and female rats. Recording at high-chart speed permits quantitative measurements of the various phases of the voiding cycle. Micturition was almost invariably associated with appearance, during a sustained increase of intravesical pressure, of a series of high-frequency oscillations, which were paralleled by a stream-like emission of the infused fluid. Micturition was not observed after topical tetrodotoxin, after bilateral transection of pelvic nerves, or in acute spinal rats. Intravenous hexamethonium produced a dose-related impairment of the voiding cycle, and, at high doses, suppressed micturition, and overflow incontinence ensued. The experimental procedure described herein appears to be suitable for physiopharmacological studies dealing with regulation of voiding cycle.
本文描述了一种新的膀胱测压程序，该程序涉及在氨基甲酸乙酯麻醉的大鼠中通过经膀胱（通过插入膀胱顶部的针）不间断输注生理盐水。这使得在雄性和雌性大鼠中都能获得一系列重复的排尿周期。以高图表速度记录允许对排尿周期的各个阶段进行定量测量。排尿几乎总是与膀胱内压持续升高期间出现的一系列高频振荡相关，这些振荡与输注液体的流状排出平行。在局部应用河豚毒素后、双侧切断盆神经后或急性脊髓大鼠中未观察到排尿。静脉注射六甲铵会导致排尿周期出现剂量相关的损害，并且在高剂量时会抑制排尿，继而出现溢流性尿失禁。本文所述的实验程序似乎适用于涉及排尿周期调节的生理药理学研究。
