Mohammadi Adibeh, Eivazzadeh-Keihan Reza, Aliabadi Hooman Aghamirza Moghim, Kashtiaray Amir, Cohan Reza Ahangari, Bani Milad Salimi, Komijani Samira, Etminan Andisheh, Salehpour Nabi, Maleki Ali, Mahdavi Mohammad
Catalysts and Organic Synthesis Research Laboratory, Department of Chemistry, Iran University of Science and Technology, Tehran 16846-13114, Iran.
Nanobiotechnology Department, New Technologies Research Group, Pasteur Institute of Iran, Tehran, Iran.
J Biotechnol. 2023 Apr 10;367:71-80. doi: 10.1016/j.jbiotec.2023.04.001. Epub 2023 Apr 5.
In this work, a magnetic nanobiocomposite scaffold based on carboxymethylcellulose (CMC) hydrogel, silk fibroin (SF), and magnetite nanoparticles was fabricated. The structural properties of this new magnetic nanobiocomposite were characterized by various analyses such as FT-IR, XRD, EDX, FE-SEM, TGA and VSM. According to the particle size histogram, most of the particles were between 55 and 77 nm and the value of saturation magnetization of this nanobiocomposite was reported 41.65 emu.g. Hemolysis and MTT tests showed that the designed magnetic nanobiocomposite was compatible with the blood. In addition, the viability percentage of HEK293T normal cells did not change significantly, and the proliferation rate of BT549 cancer cells decreased in its vicinity. EC50 values for HEK293T normal cells after 48 h and 72 h were 3958 and 2566, respectively. Also, these values for BT549 cancer cells after 48 h and 72 h were 0.4545 and 0.9967, respectively. The efficiency of fabricated magnetic nanobiocomposite was appraised in a magnetic fluid hyperthermia manner. The specific absorption rate (SAR) of 69 W/g (for the 1 mg/mL sample at 200 kHz) was measured under the alternating magnetic field (AMF).
在这项工作中，制备了一种基于羧甲基纤维素（CMC）水凝胶、丝素蛋白（SF）和磁铁矿纳米颗粒的磁性纳米生物复合支架。通过傅里叶变换红外光谱（FT-IR）、X射线衍射（XRD）、能谱分析（EDX）、场发射扫描电子显微镜（FE-SEM）、热重分析（TGA）和振动样品磁强计（VSM）等各种分析方法对这种新型磁性纳米生物复合材料的结构性能进行了表征。根据粒径直方图，大多数颗粒在55至77纳米之间，该纳米生物复合材料的饱和磁化强度值报告为41.65 emu/g。溶血和MTT试验表明，所设计的磁性纳米生物复合材料与血液相容。此外，HEK293T正常细胞的活力百分比没有显著变化，而BT549癌细胞在其附近的增殖速率降低。HEK293T正常细胞在48小时和72小时后的半数有效浓度（EC50）值分别为3958和2566。同样，BT549癌细胞在48小时和72小时后的这些值分别为0.4545和0.9967。以磁流体热疗的方式评估了制备的磁性纳米生物复合材料的效率。在交变磁场（AMF）下测量了69 W/g的比吸收率（SAR）（对于1 mg/mL样品在200 kHz时）。
