Nanobiotechnology Department, New Technologies Research Group, Pasteur Institute of Iran, Tehran, Iran.
Catalysts and Organic Synthesis Research Laboratory, Department of Chemistry, Iran University of Science and Technology, Tehran 16846-13114, Iran.
Int J Biol Macromol. 2023 Jan 1;224:1478-1486. doi: 10.1016/j.ijbiomac.2022.10.236. Epub 2022 Oct 31.
In the current study, sodium alginate (SA) and tannic acid (TA), in the presence of calcium chloride as a cross-linker, were used to fabricate a nanocomposite scaffold. With the addition of silk fibroin (SF), the strength of the synthesized composite was increased. FeO magnetic nanoparticles (MNPs) led to the usage of this magnetic nanocomposite in hyperthermia applications. Various properties of this scaffold were investigated by field emission scanning electron microscope (FE-SEM), thermogravimetric analysis (TGA), Fourier-transformed infrared (FT-IR), energy dispersive X-Ray (EDX), Vibrating- sample magnetometer (VSM). A hemolytic assay of this magnetic nanocomposite demonstrated that about 100 % of red blood cells (RBCs) survived at a concentration of 2 mg/ml, proving this scaffold is hemocompatible. Furthermore, an MTT assay was utilized to assess the cytotoxicity of the synthesized magnetic nanocomposite. Finally, the hyperthermia behavior of the fabricated magnetic nanocomposite was evaluated, and the specific absorption rate (SAR) was 73.53 W/g. The proposed nanocomposite is a good candidate for wound dressing applications in future studies.
在本研究中，使用海藻酸钠（SA）和单宁酸（TA），在氯化钙作为交联剂的存在下，制备了纳米复合支架。通过添加丝素蛋白（SF），提高了合成复合材料的强度。FeO 磁性纳米粒子（MNPs）使得这种磁性纳米复合材料能够应用于热疗。通过场发射扫描电子显微镜（FE-SEM）、热重分析（TGA）、傅里叶变换红外（FT-IR）、能谱（EDX）、振动样品磁强计（VSM）研究了这种支架的各种性质。这种磁性纳米复合材料的溶血试验表明，在 2mg/ml 的浓度下，约有 100%的红细胞（RBC）存活，证明这种支架具有良好的血液相容性。此外，还利用 MTT 法评估了合成磁性纳米复合材料的细胞毒性。最后，评价了所制备的磁性纳米复合材料的热疗行为，其比吸收率（SAR）为 73.53W/g。该纳米复合材料是未来伤口敷料应用的良好候选材料。
