Production of a magnetic nanocomposite for biological and hyperthermia applications based on chitosan-silk fibroin hydrogel incorporated with carbon nitride.

Hydrogels based on natural polymers have lightened the path of novel drug delivery systems, wound healing, and tissue engineering fields because they are renewable, non-toxic, biocompatible, and biodegradable. Furthermore, applying modified hydrogels can upgrade their biological activity. Herein, Chitosan (CS) was used to create a hydrogel using terephthaloyl thiourea as a cross-linker. Silk fibroin (SF) and carbon nitride (CN) were added to the hydrogel to enhance its strength and biocompatibility. Finally, CS hydrogel/SF/CN was in situ magnetized using FeO magnetic nanoparticles (MNPs) and manufactured as a nanobiocomposite for improved hyperthermia. The structural properties of the nanobiocomposite were assessed using several analytical techniques, including VSM, FTIR, TGA, EDX, XRD, and FESEM. The saturation magnetization of this magnetic nanocomposite was 23.94 emu/g. The hemolytic experiment on the nanobiocomposite resulted in ca. 98 % cell survival, with a hemolysis rate of 1.69 %. Anticancer property is confirmed by a 20.0 % reduction in cell viability of BT549 cells at 1.75 mg/mL concentration compared to 0.015 mg/mL. The nanocomposite is non-toxic to the human embryonic kidney cell line (HEK293T), indicating its potential for biomedical applications. Finally, the magnetic nanocomposite's hyperthermia behavior was examined using a specific absorption rate (SAR), achieving the highest value of 47.44 W/g at 200.0 kHz. When subjected to an alternating magnetic field, the nanobiocomposite may perform well in hyperthermia therapy. These results indicate that the magnetic nanobiocomposite has the potential to perform well in hyperthermia therapy when subjected to an alternating magnetic field.