Pritchard D G, Gregory R L, Michalek S M, McGhee J R
Mol Immunol. 1986 Feb;23(2):141-5. doi: 10.1016/0161-5890(86)90035-0.
The structure of the Streptococcus mutans serotype e polysaccharide was studied in order to determine the chemical basis of the immunological cross-reactions observed between it and the streptococcal group E polysaccharide. The chemical structure was established using methylation analysis, periodate oxidation, partial methanolysis and 13C NMR spectroscopy. The polysaccharide was found to consist of a polyrhamnose backbone of alternating 2- and 3-linked alpha-L-rhamnose units and sidechain beta-D-glucosyl units linked to the 2-position of rhamnose units in the backbone. This structure of the oligosaccharide repeating unit of the S. mutans serotype e polysaccharide was identical to that of the group-specific polysaccharide of group E Streptococcus. Possible explanations for the previously reported differences in these two polysaccharides are discussed.
为了确定变形链球菌血清型e多糖与E群链球菌多糖之间观察到的免疫交叉反应的化学基础，对其结构进行了研究。使用甲基化分析、高碘酸盐氧化、部分甲醇解和13C核磁共振光谱确定了化学结构。发现该多糖由交替的2-和3-连接的α-L-鼠李糖单元的聚鼠李糖主链和连接到主链中鼠李糖单元2-位的侧链β-D-葡萄糖基单元组成。变形链球菌血清型e多糖的寡糖重复单元的这种结构与E群链球菌的群特异性多糖的结构相同。讨论了先前报道的这两种多糖差异的可能解释。
